Analysis of gene expression for activin/TGFβ-signaling pathway by focused microarray
No. expressed genes/no. total genes . | Gene name . |
|---|---|
| 39/96 | ACVR2, ACVR2B, LeftyA, LeftyB, TDGF1, CER1 Stat1, TSC22, Identify, Identifying, Identified, Smad3, Smad5, Smad6, SOX4, CDC25a, Phosphatase, p21Waf1 (p21Cip1), COLIA2, COL3A1 |
| FST/Follistatin, BMP2, BMP9, ALK-3, BMP10, INH6, jun-B, DPC4, c-myc, NMA, TGIF, TIMP1, ALK-2, ALK_4, AMH, AMHR2, MADH2, TGFB1, BMPR2 |
No. expressed genes/no. total genes . | Gene name . |
|---|---|
| 39/96 | ACVR2, ACVR2B, LeftyA, LeftyB, TDGF1, CER1 Stat1, TSC22, Identify, Identifying, Identified, Smad3, Smad5, Smad6, SOX4, CDC25a, Phosphatase, p21Waf1 (p21Cip1), COLIA2, COL3A1 |
| FST/Follistatin, BMP2, BMP9, ALK-3, BMP10, INH6, jun-B, DPC4, c-myc, NMA, TGIF, TIMP1, ALK-2, ALK_4, AMH, AMHR2, MADH2, TGFB1, BMPR2 |
A subset of genes known to be part of the activin/TGFβ-signaling pathway was assessed by a focused microarray. RNA isolated from 3 different undifferentiated huES cell lines was pooled (PES), tested for the absence of markers of differentiation by RT-PCR and immunocytochemistry, and used to prepare cDNA that was used to probe for an activin receptor superfamily–focused microarray. Expression was normalized to housekeeping genes and background signal was subtracted using blanks included in the array. Experiments were performed in duplicate with independently isolated samples and similar results were obtained with other cell lines (data not shown).