EST enumeration was done as described in R.B. et al (unpublished data, August 2003). Columns ES and EB indicate the number of ESTs found for a particular gene. The Fisher exact test (P ≤ .05 was used to determine whether the difference in EST number is likely to reflect a real difference in expression levels. OE indicates a gene overexpressed in the ES cells. For lower copy number (5 ESTs and below in ES and 0 in EB, or less than 7 ESTs in ES and 1 in EB) the test cannot determine significance, even though the differences might be real. The signal of all genes was ≥ 3-fold higher compared with huURNA in microarray experiments.

Genes significantly overexpressing ES compared with EB.