Expression profiling of 6 huES cell lines
Category* . | Common genes expressed in all 6 huES cell lines† . | Genes overexpressed but not in all 6 cell lines‡ . |
|---|---|---|
| Markers of undifferentiated huES cell lines (6) | GJA 1, Podocalyxin-like (GTCM-1), Nanog, galanin§, Oct 4 (POU5F1), GDF3§ | SOX2, DNMT3B, ACVR2B |
| Differentiation markers (5) | Keratin 8,∥ Keratin 18,∥ ACTC,∥ TUBB-5,∥ TNNT 1∥ | |
| Cell signaling/cell cycle/cell growth/cellular process (19) | PSIP1§, SFRP2, TDGF1, Sema6a, CRABP1, CRABP2, NME2, IMP-2, CCNB1, LEFTB, Calumenin, CDC2, LIN-28, MAD2L2, PITX2§, STK12, PTTG1, SET, BRIX | CD24, CER1 |
| Metabolism: DNA and RNA related (21) | RPS24, RPL4, RPL6, RPL7, RPLP0, RPL24, NBR2, HDAC2, HMGIY, HMGB2, KPNA2, SNRPF, DDX21, Nucleostemin, SSB, HNRAPB, RAMP, EPRS, EIF4A1, NPM1, JADE-1 | |
| Metabolic pathways (23) | SMS, SPS, CYP26A1, HSPA4, ELOVL6, FABP5, IMPDH2, IDH1, LDHB, LAPTM4B, MGST1, CCNC, MTHFD1, MTHFD2, TK1, PSMA2, PSMA3, HSSG1, SERPINH1, SLC 16A1, KIF4A, CCT8, TUBB4 | |
| Others (3) | NASP, DSG2, LRRN1 | |
| Zinc finger protein (3) | Zinc finger protein 43, znf 117, znf 257 | |
| Hypothetical protein (12) | KIAA 1573, MGC27165, GSH1§, C20orf1, C15orf15, C20orf129, TD60, HNRPA1, ARL8, Ribosomal s40A lamin R, Numatrin, PPAT |
Category* . | Common genes expressed in all 6 huES cell lines† . | Genes overexpressed but not in all 6 cell lines‡ . |
|---|---|---|
| Markers of undifferentiated huES cell lines (6) | GJA 1, Podocalyxin-like (GTCM-1), Nanog, galanin§, Oct 4 (POU5F1), GDF3§ | SOX2, DNMT3B, ACVR2B |
| Differentiation markers (5) | Keratin 8,∥ Keratin 18,∥ ACTC,∥ TUBB-5,∥ TNNT 1∥ | |
| Cell signaling/cell cycle/cell growth/cellular process (19) | PSIP1§, SFRP2, TDGF1, Sema6a, CRABP1, CRABP2, NME2, IMP-2, CCNB1, LEFTB, Calumenin, CDC2, LIN-28, MAD2L2, PITX2§, STK12, PTTG1, SET, BRIX | CD24, CER1 |
| Metabolism: DNA and RNA related (21) | RPS24, RPL4, RPL6, RPL7, RPLP0, RPL24, NBR2, HDAC2, HMGIY, HMGB2, KPNA2, SNRPF, DDX21, Nucleostemin, SSB, HNRAPB, RAMP, EPRS, EIF4A1, NPM1, JADE-1 | |
| Metabolic pathways (23) | SMS, SPS, CYP26A1, HSPA4, ELOVL6, FABP5, IMPDH2, IDH1, LDHB, LAPTM4B, MGST1, CCNC, MTHFD1, MTHFD2, TK1, PSMA2, PSMA3, HSSG1, SERPINH1, SLC 16A1, KIF4A, CCT8, TUBB4 | |
| Others (3) | NASP, DSG2, LRRN1 | |
| Zinc finger protein (3) | Zinc finger protein 43, znf 117, znf 257 | |
| Hypothetical protein (12) | KIAA 1573, MGC27165, GSH1§, C20orf1, C15orf15, C20orf129, TD60, HNRPA1, ARL8, Ribosomal s40A lamin R, Numatrin, PPAT |
Numbers in parentheses represent the number of genes under each category.
A common set of genes (n = 92) elevated in all 6 huES cell lines (TE06, GE01, GE09, BG01, BG02, and PES) at a 99% confidence interval (3-fold or higher) are shown.
ES cell-specific genes were not shown to be expressed (≥ 3-fold) in all 6 huES cell lines.
These genes showed no expression by EST enumeration.
These genes are not expected to be present in undifferentiated ES cells because they are known differentiation markers and we used undifferentiated cells.