Expression levels in ES cells infected with Pim-1 RNAi versus Flk1+/+ ES cells at 5.5 and 8.5 days of differentiation in vitro
. | Fold increase in Pim-1 RNAi . | . | |
|---|---|---|---|
| Gene . | Day 5.5 . | Day 8.5 . | |
| Flk-1 | −2.7 ± 0.4 | −14.2 ± 21 | |
| PECAM1 | −2.8 ± 0.3 | −4.5 ± 0.5 | |
| VE-cadherin | −8.8 ± 1.2 | −42 ± 6.3 | |
| Tie-2 | −2.9 ± 0.3 | −35.0 ± 4.4 | |
| Flt4 | −5.9 ± 1.0 | −1.4 ± 0.1 | |
| VEGF-A | −12.3 ± 1.1 | −1.3 ± 0.1 | |
| VEGF-D | −1.6 ± 0.2 | −2.5 ± 0.4 | |
| α-SMA | −4.6 ± 0.6 | −2.5 ± 0.3 | |
| SM22α | −4.5 ± 0.5 | −2.6 ± 0.4 | |
| Calponin-h1 | −7.3 ± 0.9 | −1.7 ± 0.2 | |
| SM-MHC | −3.7 ± 0.3 | +1.7 ± 0.1 | |
| Brachyury | −37.3 ± 4.9 | +1.0 ± 0.1 | |
| α 1-fetoprotein | −1.1 ± 0.1 | +143 ± 18 | |
| Nestin | +1.4 ± 0.2 | −3.5 ± 0.5 | |
| Myogenin | +81.2 ± 11 | +129.0 ± 15 | |
. | Fold increase in Pim-1 RNAi . | . | |
|---|---|---|---|
| Gene . | Day 5.5 . | Day 8.5 . | |
| Flk-1 | −2.7 ± 0.4 | −14.2 ± 21 | |
| PECAM1 | −2.8 ± 0.3 | −4.5 ± 0.5 | |
| VE-cadherin | −8.8 ± 1.2 | −42 ± 6.3 | |
| Tie-2 | −2.9 ± 0.3 | −35.0 ± 4.4 | |
| Flt4 | −5.9 ± 1.0 | −1.4 ± 0.1 | |
| VEGF-A | −12.3 ± 1.1 | −1.3 ± 0.1 | |
| VEGF-D | −1.6 ± 0.2 | −2.5 ± 0.4 | |
| α-SMA | −4.6 ± 0.6 | −2.5 ± 0.3 | |
| SM22α | −4.5 ± 0.5 | −2.6 ± 0.4 | |
| Calponin-h1 | −7.3 ± 0.9 | −1.7 ± 0.2 | |
| SM-MHC | −3.7 ± 0.3 | +1.7 ± 0.1 | |
| Brachyury | −37.3 ± 4.9 | +1.0 ± 0.1 | |
| α 1-fetoprotein | −1.1 ± 0.1 | +143 ± 18 | |
| Nestin | +1.4 ± 0.2 | −3.5 ± 0.5 | |
| Myogenin | +81.2 ± 11 | +129.0 ± 15 | |
Data are presented as mean ± SEM. The fold change detected by quantitative RT-PCR represents the average among the results of 4 independent measurements.