Comparison of the expression levels of transcripts differentially expressed in Flk1+/+ versus Flk1−/− ES cells at 5.5 days of differentiation in vitro by macroarray analysis and real-time quantitative RT-PCR
Category/gene . | Fold increase by macroarray . | Fold increase by RT-PCR . |
|---|---|---|
| Nuclear factor | ||
| Cbp | 4.3 ± 0.6 | 7.6 ± 0.5 |
| Hec | 6.4 ± 1.2 | 3.4 ± 0.2 |
| Nm23M1 | 3.1 ± 0.8 | 3.8 ± 0.7 |
| Nm23M2 | 3.9 ± 0.6 | 2.1 ± 0.3 |
| Pim-1 | 2.9 ± 0.9 | 3.2 ± 0.2 |
| pp32 | 3.7 ± 0.8 | 3.2 ± 0.5 |
| Set | 5.2 ± 0.8 | 2.4 ± 0.2 |
| Slug | 3.4 ± 0.3 | 5.9 ± 0.5 |
| Sox18 | 5.7 ± 0.2 | 4.4 ± 0.7 |
| Growth factor | ||
| Sfrp1 (FrzA) | 4.9 ± 0.3 | 11.3 ± 1.2 |
| Receptors | ||
| Edg5 | 4.6 ± 0.3 | 3.1 ± 0.5 |
| EphB3 (Sek4) | 3.5 ± 0.2 | 2.2 ± 0.3 |
| Signal transduction | ||
| Ship-1 | 9.9 ± 0.8 | 7.0 ± 0.4 |
| Btk | 3.0 ± 0.3 | 3.2 ± 0.2 |
| Unknown | ||
| Mesdc2 | > 20 | 13.6 ± 3.1 |
Category/gene . | Fold increase by macroarray . | Fold increase by RT-PCR . |
|---|---|---|
| Nuclear factor | ||
| Cbp | 4.3 ± 0.6 | 7.6 ± 0.5 |
| Hec | 6.4 ± 1.2 | 3.4 ± 0.2 |
| Nm23M1 | 3.1 ± 0.8 | 3.8 ± 0.7 |
| Nm23M2 | 3.9 ± 0.6 | 2.1 ± 0.3 |
| Pim-1 | 2.9 ± 0.9 | 3.2 ± 0.2 |
| pp32 | 3.7 ± 0.8 | 3.2 ± 0.5 |
| Set | 5.2 ± 0.8 | 2.4 ± 0.2 |
| Slug | 3.4 ± 0.3 | 5.9 ± 0.5 |
| Sox18 | 5.7 ± 0.2 | 4.4 ± 0.7 |
| Growth factor | ||
| Sfrp1 (FrzA) | 4.9 ± 0.3 | 11.3 ± 1.2 |
| Receptors | ||
| Edg5 | 4.6 ± 0.3 | 3.1 ± 0.5 |
| EphB3 (Sek4) | 3.5 ± 0.2 | 2.2 ± 0.3 |
| Signal transduction | ||
| Ship-1 | 9.9 ± 0.8 | 7.0 ± 0.4 |
| Btk | 3.0 ± 0.3 | 3.2 ± 0.2 |
| Unknown | ||
| Mesdc2 | > 20 | 13.6 ± 3.1 |
Data are presented as mean ± SEM. The fold change detected by quantitative RT-PCR represents the average between the results of 3 independent experiments.