Expression of microparticle-associated tissue factor procoagulant activity on in vitro-generated microparticles
Cellular origin of MPs . | MP-associated TF PCA in supernatant [Xa], pM/h per 106 cells . |
|---|---|
| Fibroblasts | |
| Ionophore− | > 300 |
| Ionophore+ | > 300 |
| Smooth muscle cells | |
| Ionophore− | > 300 |
| Ionophore+ | > 300 |
| HUVECs | |
| TNF− | 4 |
| TNF+ | 26 |
| CD14+ monocytes | |
| LPS− | 3 |
| LPS+ | 20 |
| Platelets | |
| Ionophore− | 0 |
| Ionophore+ | 0 |
| Red blood cells | |
| Ionophore− | 0 |
| Ionophore+ | 1 |
Cellular origin of MPs . | MP-associated TF PCA in supernatant [Xa], pM/h per 106 cells . |
|---|---|
| Fibroblasts | |
| Ionophore− | > 300 |
| Ionophore+ | > 300 |
| Smooth muscle cells | |
| Ionophore− | > 300 |
| Ionophore+ | > 300 |
| HUVECs | |
| TNF− | 4 |
| TNF+ | 26 |
| CD14+ monocytes | |
| LPS− | 3 |
| LPS+ | 20 |
| Platelets | |
| Ionophore− | 0 |
| Ionophore+ | 0 |
| Red blood cells | |
| Ionophore− | 0 |
| Ionophore+ | 1 |
Cells were isolated as described in “Materials and methods.” Fibroblasts, smooth muscle cells, red blood cells, and platelets (106 cells in each case) were activated by exposure to 10 μM ionophore (or vehicle control) for 10 minutes at 37°C. Monocytes were activated by exposure to 10 ng/mL LPS (or vehicle control) for 4 hours at 37°C and HUVECs were activated by exposure to 10 ng/mL TNF-α (or vehicle control) for 4 hours at 37°C. In every case, the supernatant was removed and assayed for MP-associated TF PCA as described in “Materials and methods.”