Table 1.

Summary of mouse transplantation experiments

Experiment and mouse no.
cGy
Cell dose, × 106
CFC transduction, %
% GFP-positive RBCs at 12 wks
% GFP-positive RBCs at 24 wks
% GFP-positive platelets at 24 wks
Treated with CID
Response to CID
Comments/adverse events
1          
2160   400   10   57   29.63   25.26   11.12   No   —   Killed wk 37  
2161   400   10   57   37.73   35.08   12.15   No   —   Killed wk 37  
2162   400   10   57   35.67   27.25   6.62   No   —   Killed wk 37  
2168   100   10   57   0.11   0.1   0.1   No   —   Killed wk 37  
2172   100   10   57   0.12   0.1   0.09   No   —   Killed wk 37  
2          
2781   400   2   49   0.07   0.05   0.01   Yes   No   Killed wk 41  
2782   400   2   49   4.15   0.13   0.02   No   —   Killed wk 41  
2778   400   2   49   4.51   0.07   0.03   No   —   Killed wk 41  
2779   400   2   49   1.47   0.04   0.03   No   —   Killed wk 41  
2785   400   4   49   8.79   48.75*  1.85   Yes   Yes   Killed wk 45  
2752   400   4   49   10.84   12.41   2.91   No   —   Killed wk 45  
2777   400   4   49   7.01   —   —   —   —   Died after single CID dose, wk 17  
2780   400   4   49   8.21   12.96   1.51   No   —   Died wk 40  
3          
2407   400   5   55   15.72   19.94   2.35   No   —   Killed wk 67  
2408   400   5   55   10.29   7.52   1.09   Yes   Yes   Died wk 50  
2409   400   5   55   8.53   8.59   1.01   Yes   Yes   Killed wk 67  
2410   400   5   55   9.98   4.44   0.83   No   —   Killed wk 67  
2411   400   5   55   11.71   11.81   2.65   Yes   Yes   Killed wk 67  
2412   400   5   55   19.12   24.02   6.07   No   —   Killed wk 67  
2413   400   5   55   17.13   22.17   3.97   Yes†  Yes†  Killed wk 67; CID wk 63  
2414   400   5   55   10.21   8.97   1.88   Yes   Yes   Died wk 48  
2415   400   5   55   14.58   7.46   2.72   Yes   Yes   Died wk 62  
2416   400   5   55   13.5   17.27   3.13   No   —   Died wk 39  
4          
1674   0   5   49   0   —   —   No   —   Killed wk 50  
1246   0   5   49   0   —   —   Yes   No   Killed wk 50  
1247   0   5   49   0   —   —   Yes   No   Died  
1675   0   5   49   0   —   —   Yes   No   Killed wk 50  
5          
2952   400   10   66.7   55.42   47.84   7.46   Yes   Yes   Died wk 110  
2953   400   10   66.7   40.6   53.02   8.47   Yes   Yes   Died wk 123  
2954   400   < 2   66.7   5.76   5.25   0.13   No   —   Died wk 124  
6          
2891   100   2   66   2.89   0.96   0.08   Yes   No   Killed wk 48  
2163   100   5   66   2.25   0.75   0.05   No   —   Died wk 47  
2165
 		 100
 		 10
 		 66
 		 5.21
 		 5.25
 		 0.4
 		 Yes
 		 Yes
 		 Died wk 33
 
Experiment and mouse no.
cGy
Cell dose, × 106
CFC transduction, %
% GFP-positive RBCs at 12 wks
% GFP-positive RBCs at 24 wks
% GFP-positive platelets at 24 wks
Treated with CID
Response to CID
Comments/adverse events
1          
2160   400   10   57   29.63   25.26   11.12   No   —   Killed wk 37  
2161   400   10   57   37.73   35.08   12.15   No   —   Killed wk 37  
2162   400   10   57   35.67   27.25   6.62   No   —   Killed wk 37  
2168   100   10   57   0.11   0.1   0.1   No   —   Killed wk 37  
2172   100   10   57   0.12   0.1   0.09   No   —   Killed wk 37  
2          
2781   400   2   49   0.07   0.05   0.01   Yes   No   Killed wk 41  
2782   400   2   49   4.15   0.13   0.02   No   —   Killed wk 41  
2778   400   2   49   4.51   0.07   0.03   No   —   Killed wk 41  
2779   400   2   49   1.47   0.04   0.03   No   —   Killed wk 41  
2785   400   4   49   8.79   48.75*  1.85   Yes   Yes   Killed wk 45  
2752   400   4   49   10.84   12.41   2.91   No   —   Killed wk 45  
2777   400   4   49   7.01   —   —   —   —   Died after single CID dose, wk 17  
2780   400   4   49   8.21   12.96   1.51   No   —   Died wk 40  
3          
2407   400   5   55   15.72   19.94   2.35   No   —   Killed wk 67  
2408   400   5   55   10.29   7.52   1.09   Yes   Yes   Died wk 50  
2409   400   5   55   8.53   8.59   1.01   Yes   Yes   Killed wk 67  
2410   400   5   55   9.98   4.44   0.83   No   —   Killed wk 67  
2411   400   5   55   11.71   11.81   2.65   Yes   Yes   Killed wk 67  
2412   400   5   55   19.12   24.02   6.07   No   —   Killed wk 67  
2413   400   5   55   17.13   22.17   3.97   Yes†  Yes†  Killed wk 67; CID wk 63  
2414   400   5   55   10.21   8.97   1.88   Yes   Yes   Died wk 48  
2415   400   5   55   14.58   7.46   2.72   Yes   Yes   Died wk 62  
2416   400   5   55   13.5   17.27   3.13   No   —   Died wk 39  
4          
1674   0   5   49   0   —   —   No   —   Killed wk 50  
1246   0   5   49   0   —   —   Yes   No   Killed wk 50  
1247   0   5   49   0   —   —   Yes   No   Died  
1675   0   5   49   0   —   —   Yes   No   Killed wk 50  
5          
2952   400   10   66.7   55.42   47.84   7.46   Yes   Yes   Died wk 110  
2953   400   10   66.7   40.6   53.02   8.47   Yes   Yes   Died wk 123  
2954   400   < 2   66.7   5.76   5.25   0.13   No   —   Died wk 124  
6          
2891   100   2   66   2.89   0.96   0.08   Yes   No   Killed wk 48  
2163   100   5   66   2.25   0.75   0.05   No   —   Died wk 47  
2165
 		 100
 		 10
 		 66
 		 5.21
 		 5.25
 		 0.4
 		 Yes
 		 Yes
 		 Died wk 33
 

Experiment 1 is depicted in Figure 2; experiment 2 in Figure 3; and experiment 3 in Figures 4, 5, 6. Experiments 4 to 6 are not depicted in the figures. CFC indicates colony-forming cells.—indicates that testing was not performed.

*

Mouse 2785 was treated at week 17 with CID, resulting in an elevation in the percentage of GFP-positive RBCs.

†

Mouse 2413 did not receive CID until week 63 after transplantation and is therefore included with the nontreated control mice in Figures 4 and 5.

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