CD34 clones from peripheral blood and bone marrow and single granulocytes for mtDNA analysis, successfully assayed by nested PCR for mtDNA sequence
. | Control region . | . | . | . | . | . | . | . | . | . | . | Coding region (CO1 and Cytb) . | . | . | . | . | . | . | . | . | . | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor (age, y/sex) . | BM CD34 clone*, no. . | . | . | . | . | PB CD34 clone, no. . | . | . | . | . | Single granulocyte, no. . | BM CD34 clone, no. . | . | . | . | . | PB CD34 clone, no. . | . | . | . | . | ||||||||||||||||||
| . | G1 . | G2 . | G3 . | G4 . | Subtotal . | G1 . | G2 . | G3 . | G4 . | Subtotal . | . | G1 . | G2 . | G3 . | G4 . | Subtotal . | G1 . | G2 . | G3 . | G4 . | Subtotal . | ||||||||||||||||||
| 1 (47/F) | 28 | 20 | 30 | 37 | 115 | 13 | 30 | 47 | 30 | 120 | 51 | 28 | 20 | 29 | 17 | 94 | 13 | 30 | 35 | 17 | 95 | ||||||||||||||||||
| 2 (38/F) | 23 | 3 | 4 | 6 | 36 | 23 | 24 | 23 | 24 | 94 | 37 | — | — | — | — | — | — | — | — | — | — | ||||||||||||||||||
| 3 (43/M) | 30 | 30 | 30 | 30 | 120 | 24 | 20 | 23 | 26 | 93 | 50 | 25 | 26 | 24 | 20 | 95 | 24 | 20 | 23 | 26 | 93 | ||||||||||||||||||
| 4 (34/M) | 26 | 28 | 28 | 29 | 111 | 23 | 24 | 24 | 24 | 95 | 111 | — | — | — | — | — | — | — | — | — | — | ||||||||||||||||||
| 5 (54/M) | 26 | 28 | 30 | 30 | 114 | 24 | 24 | 24 | 24 | 96 | 42 | 25 | 29 | 30 | 12 | 96 | 24 | 24 | 24 | 24 | 96 | ||||||||||||||||||
| 6 (34/F) | 25 | 30 | 30 | 30 | 115 | 24 | 24 | 24 | 24 | 96 | 64 | — | — | — | — | — | — | — | — | — | — | ||||||||||||||||||
| Total | 158 | 139 | 152 | 162 | 611 | 131 | 146 | 165 | 152 | 594 | 355 | 78 | 75 | 83 | 49 | 285 | 61 | 74 | 82 | 67 | 284 | ||||||||||||||||||
. | Control region . | . | . | . | . | . | . | . | . | . | . | Coding region (CO1 and Cytb) . | . | . | . | . | . | . | . | . | . | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor (age, y/sex) . | BM CD34 clone*, no. . | . | . | . | . | PB CD34 clone, no. . | . | . | . | . | Single granulocyte, no. . | BM CD34 clone, no. . | . | . | . | . | PB CD34 clone, no. . | . | . | . | . | ||||||||||||||||||
| . | G1 . | G2 . | G3 . | G4 . | Subtotal . | G1 . | G2 . | G3 . | G4 . | Subtotal . | . | G1 . | G2 . | G3 . | G4 . | Subtotal . | G1 . | G2 . | G3 . | G4 . | Subtotal . | ||||||||||||||||||
| 1 (47/F) | 28 | 20 | 30 | 37 | 115 | 13 | 30 | 47 | 30 | 120 | 51 | 28 | 20 | 29 | 17 | 94 | 13 | 30 | 35 | 17 | 95 | ||||||||||||||||||
| 2 (38/F) | 23 | 3 | 4 | 6 | 36 | 23 | 24 | 23 | 24 | 94 | 37 | — | — | — | — | — | — | — | — | — | — | ||||||||||||||||||
| 3 (43/M) | 30 | 30 | 30 | 30 | 120 | 24 | 20 | 23 | 26 | 93 | 50 | 25 | 26 | 24 | 20 | 95 | 24 | 20 | 23 | 26 | 93 | ||||||||||||||||||
| 4 (34/M) | 26 | 28 | 28 | 29 | 111 | 23 | 24 | 24 | 24 | 95 | 111 | — | — | — | — | — | — | — | — | — | — | ||||||||||||||||||
| 5 (54/M) | 26 | 28 | 30 | 30 | 114 | 24 | 24 | 24 | 24 | 96 | 42 | 25 | 29 | 30 | 12 | 96 | 24 | 24 | 24 | 24 | 96 | ||||||||||||||||||
| 6 (34/F) | 25 | 30 | 30 | 30 | 115 | 24 | 24 | 24 | 24 | 96 | 64 | — | — | — | — | — | — | — | — | — | — | ||||||||||||||||||
| Total | 158 | 139 | 152 | 162 | 611 | 131 | 146 | 165 | 152 | 594 | 355 | 78 | 75 | 83 | 49 | 285 | 61 | 74 | 82 | 67 | 284 | ||||||||||||||||||
Numbers in columns indicate CD34 clones and individual granulocytes successfully subjected to nested PCR for mtDNA sequences from the control and the CO1 and Cytb coding regions. G indicates grade; CO1, cytochrome c oxidase 1; Cytb, cytochrome b; and—, not applicable.
The same CD34 clones are used as in our recent publication.13