Absolute number and percentage of Lin− Sca1+ c-kit+ cells in the bone marrow
. | Lin− cells . | . | Scal+ c-kit+ . | . | ||
---|---|---|---|---|---|---|
Genotype . | Percentage of femur . | Absolute number, × 105 . | Percentage of lin− compartment . | Absolute number, × 104 . | ||
Wild-type | 0.48 ± 0.12 | 2.01 ± 0.50 | 11.58 ± 1.62 | 2.18 ± 0.45 | ||
Pten+/− | 0.87 ± 0.05 | 4.00 ± 0.80 | 8.37 ± 1.37 | 3.45 ± 1.21 | ||
SHIP−/− | 0.91 ± 0.24 | 2.48 ± 0.53 | 14.46 ± 3.12 | 3.58 ± 0.97 | ||
Pten+/−SHIP−/− | 0.58 ± 0.15 | 1.30 ± 0.34 | 13.35 ± 1.17 | 1.65 ± 0.37 |
. | Lin− cells . | . | Scal+ c-kit+ . | . | ||
---|---|---|---|---|---|---|
Genotype . | Percentage of femur . | Absolute number, × 105 . | Percentage of lin− compartment . | Absolute number, × 104 . | ||
Wild-type | 0.48 ± 0.12 | 2.01 ± 0.50 | 11.58 ± 1.62 | 2.18 ± 0.45 | ||
Pten+/− | 0.87 ± 0.05 | 4.00 ± 0.80 | 8.37 ± 1.37 | 3.45 ± 1.21 | ||
SHIP−/− | 0.91 ± 0.24 | 2.48 ± 0.53 | 14.46 ± 3.12 | 3.58 ± 0.97 | ||
Pten+/−SHIP−/− | 0.58 ± 0.15 | 1.30 ± 0.34 | 13.35 ± 1.17 | 1.65 ± 0.37 |
Whole bone marrow was enumerated, lineage cell depleted, recounted, and then stained for cell-surface Scal and c-kit expression. Values in the chart were extrapolated from femoral cell counts. Results represent pooled data from 3 to 4 mice per genotype ± SEM.