Table 3.

Absolute number and percentage of Lin Sca1+ c-kit+ cells in the bone marrow



Lin cells

Scal+ c-kit+
Genotype
Percentage of femur
Absolute number, × 105
Percentage of lin compartment
Absolute number, × 104
Wild-type  0.48 ± 0.12   2.01 ± 0.50   11.58 ± 1.62   2.18 ± 0.45  
Pten+/−  0.87 ± 0.05   4.00 ± 0.80   8.37 ± 1.37   3.45 ± 1.21  
SHIP−/−  0.91 ± 0.24   2.48 ± 0.53   14.46 ± 3.12   3.58 ± 0.97  
Pten+/−SHIP−/−
 
0.58 ± 0.15
 
1.30 ± 0.34
 
13.35 ± 1.17
 
1.65 ± 0.37
 


Lin cells

Scal+ c-kit+
Genotype
Percentage of femur
Absolute number, × 105
Percentage of lin compartment
Absolute number, × 104
Wild-type  0.48 ± 0.12   2.01 ± 0.50   11.58 ± 1.62   2.18 ± 0.45  
Pten+/−  0.87 ± 0.05   4.00 ± 0.80   8.37 ± 1.37   3.45 ± 1.21  
SHIP−/−  0.91 ± 0.24   2.48 ± 0.53   14.46 ± 3.12   3.58 ± 0.97  
Pten+/−SHIP−/−
 
0.58 ± 0.15
 
1.30 ± 0.34
 
13.35 ± 1.17
 
1.65 ± 0.37
 

Whole bone marrow was enumerated, lineage cell depleted, recounted, and then stained for cell-surface Scal and c-kit expression. Values in the chart were extrapolated from femoral cell counts. Results represent pooled data from 3 to 4 mice per genotype ± SEM.

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