Effect of rhHAPO on colony formation of human fetal bone marrow progenitor cells in response to cytokine stimulation
. | Colony types, no. . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Additions . | CFU-M . | CFU-G . | CFU-GM . | BFU-E . | CFU-Mix . | ||||
| PBS | 3.5 ± 0.5 | 0 | 4.5 ± 0.2 | 0 | 0 | ||||
| HAPO | 3.5 ± 0.2 | 0 | 6.5 ± 2.2 | 0 | 11 ± 2.58* | ||||
| CC | 10 ± 1.41 | 0.5 ± 0.5 | 55 ± 4.5 | 6.5 ± 1.15 | 17.5 ± 1.26 | ||||
| CC + HAPO | 13 ± 1.29 | 1.5 ± 0.57 | 67.5 ± 6.5 | 8.2 ± 2.5 | 36.75 ± 6.94† | ||||
. | Colony types, no. . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Additions . | CFU-M . | CFU-G . | CFU-GM . | BFU-E . | CFU-Mix . | ||||
| PBS | 3.5 ± 0.5 | 0 | 4.5 ± 0.2 | 0 | 0 | ||||
| HAPO | 3.5 ± 0.2 | 0 | 6.5 ± 2.2 | 0 | 11 ± 2.58* | ||||
| CC | 10 ± 1.41 | 0.5 ± 0.5 | 55 ± 4.5 | 6.5 ± 1.15 | 17.5 ± 1.26 | ||||
| CC + HAPO | 13 ± 1.29 | 1.5 ± 0.57 | 67.5 ± 6.5 | 8.2 ± 2.5 | 36.75 ± 6.94† | ||||
CC, the appropriate cocktail of cytokines, included rhIL-3 (10 ng/mL), rhGM-CSF (10 ng/mL), rhSCF (50 ng/mL), and rhEPO (3 U/mL). The rhHAPO was added at 100 ng/mL final concentration. Data are pooled from 3 separate experiments and expressed as mean ± SD. Bone marrow cells were plated a cell density of 1 × 105/35 mm dish in a methycellulose system as described in “Materials and methods.”
CFU indicates colony-forming unit; M, macrophage; G, granulocyte; BFU-E, eruthroblast burst-forming unit; Mix, mixed colony; PBS, phosphate-buffered saline.
P < .05 versus PBS.
P < .05 versus CC group.