Quantitative RT-PCR
. | Hoxb4 Ter119-depleted fetal liver . | . | . | Hoxb3/b4 Ter119-depleted fetal liver . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene . | wt; n = 9 . | b4-/-; n = 10-14 . | FD . | wt; n = 8 . | b3/b4-/-; n = 5-9 . | FD . | ||||
| HoxA4 | 0.29 ± 0.12 | 0.38 ± 0.10 | +1.3 | 0.29 ± 0.12 | 0.51 ± 0.17 | +1.7 | ||||
| HoxA9 | 3.28 ± 1.67 | 2.13 ± 2.10 | -1.5 | 2.76 ± 1.09 | 0.46 ± 0.83 | -6.0† | ||||
| HoxB2 | 2.80 ± 1.72 | 0.72 ± 0.44 | -3.9† | 2.85 ± 0.74 | 0.76 ± 0.23 | -3.8† | ||||
| HoxB3 | 1.68 ± 0.65 | 0.19 ± 0.39 | -8.8† | — | — | — | ||||
| HoxB5 | 2.33 ± 0.68 | 0.15 ± 0.13 | -16‡ | 2.25 ± 0.64 | 0.48 ± 0.25 | -4.7† | ||||
| HoxC4 | VL | VL | — | VL | VL | — | ||||
| HoxD4 | VL | VL | — | VL | VL | — | ||||
| p21 | 1.31 ± 0.80 | 2.13 ± 0.75 | +1.6* | 1.24 ± 0.74 | 2.97 ± 1.65 | +2.4* | ||||
| c-myc | 2.11 ± 2.10 | 3.92 ± 1.33 | +1.9* | 2.11 ± 1.11 | 5.67 ± 3.29 | +2.7 | ||||
| Jun-B | 3.56 ± 0.94 | 0.75 ± 0.69 | -4.7* | 3.55 ± 0.93 | 1.16 ± 0.91 | -3.1* | ||||
| CyclinD1 | 1.57 ± 0.55 | 0.43 ± 0.28 | -3.6‡ | 1.57 ± 0.54 | 0.81 ± 0.53 | -1.9* | ||||
| c-Jun | 0.41 ± 0.27 | 0.21 ± 0.14 | -2.0 | ND | ND | — | ||||
| CXCR4 | 1.75 ± 0.99 | 0.94 ± 0.77 | -1.9 | 1.69 ± 0.89 | 1.50 ± 0.97 | -1.1 | ||||
. | Hoxb4 Ter119-depleted fetal liver . | . | . | Hoxb3/b4 Ter119-depleted fetal liver . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene . | wt; n = 9 . | b4-/-; n = 10-14 . | FD . | wt; n = 8 . | b3/b4-/-; n = 5-9 . | FD . | ||||
| HoxA4 | 0.29 ± 0.12 | 0.38 ± 0.10 | +1.3 | 0.29 ± 0.12 | 0.51 ± 0.17 | +1.7 | ||||
| HoxA9 | 3.28 ± 1.67 | 2.13 ± 2.10 | -1.5 | 2.76 ± 1.09 | 0.46 ± 0.83 | -6.0† | ||||
| HoxB2 | 2.80 ± 1.72 | 0.72 ± 0.44 | -3.9† | 2.85 ± 0.74 | 0.76 ± 0.23 | -3.8† | ||||
| HoxB3 | 1.68 ± 0.65 | 0.19 ± 0.39 | -8.8† | — | — | — | ||||
| HoxB5 | 2.33 ± 0.68 | 0.15 ± 0.13 | -16‡ | 2.25 ± 0.64 | 0.48 ± 0.25 | -4.7† | ||||
| HoxC4 | VL | VL | — | VL | VL | — | ||||
| HoxD4 | VL | VL | — | VL | VL | — | ||||
| p21 | 1.31 ± 0.80 | 2.13 ± 0.75 | +1.6* | 1.24 ± 0.74 | 2.97 ± 1.65 | +2.4* | ||||
| c-myc | 2.11 ± 2.10 | 3.92 ± 1.33 | +1.9* | 2.11 ± 1.11 | 5.67 ± 3.29 | +2.7 | ||||
| Jun-B | 3.56 ± 0.94 | 0.75 ± 0.69 | -4.7* | 3.55 ± 0.93 | 1.16 ± 0.91 | -3.1* | ||||
| CyclinD1 | 1.57 ± 0.55 | 0.43 ± 0.28 | -3.6‡ | 1.57 ± 0.54 | 0.81 ± 0.53 | -1.9* | ||||
| c-Jun | 0.41 ± 0.27 | 0.21 ± 0.14 | -2.0 | ND | ND | — | ||||
| CXCR4 | 1.75 ± 0.99 | 0.94 ± 0.77 | -1.9 | 1.69 ± 0.89 | 1.50 ± 0.97 | -1.1 | ||||
cDNA was from Ter119-depleted day-14.5 fetal liver cells from wt and Hoxb4-/- and Hoxb3/b4-/- mice. HPRT was used to normalize cDNA concentration for each PCR product.
Values are presented as mean ± SD for the relative intensity of cDNA from the indicated gene compared with HPRT. FD indicates fold difference; — indicates not applicable; VL, very low levels, not or barely detectable; and ND, not done.
P < .05
P ≤ .01
P ≤ .001