T-lymphocyte recovery in spleen after congenic purified hematopoietic stem cell transplantation (day + 42)
. | Groups . | . | . | P . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|---|
. | 400 KTLS . | 1000 KTLS . | 5000 KTLS . | 400 vs 1000 . | 400 vs 5000 . | 1000 vs 5000 . | ||||
n | 4 | 7 | 4 | — | — | — | ||||
Total | ||||||||||
CD3+ | 19.6 ± 4.3 | 28.5 ± 5.6 | 30.4 ± 2.9 | .0115 | .0076 | .5369 | ||||
CD4+ | 11.3 ± 3.0 | 18.0 ± 3.4 | 18.9 ± 1.2 | .0032 | .0029 | .6087 | ||||
CD8+ | 6.3 ± 2.3 | 8.3 ± 2.2 | 7.3 ± 2.2 | .1814 | .5309 | .5028 | ||||
Naive CD4+ | 8.5 ± 3.5 | 14.4 ± 2.9 | 15.3 ± 1.3 | .0054 | .0049 | .6346 | ||||
Naive CD8+ | 0.9 ± 0.5 | 2.7 ± 1.0 | 3.4 ± 0.8 | .0088 | .0018 | .1941 | ||||
Donor-type | ||||||||||
CD3+ | 5.3 ± 2.3 | 11.8 ± 3.6 | 13.0 ± 0.6 | .0032 | .0023 | .5197 | ||||
CD4+ | 3.2 ± 1.6 | 7.5 ± 2.3 | 7.3 ± 0.4 | .0026 | .0079 | .8462 | ||||
CD8+ | 0.9 ± 0.5 | 2.6 ± 1.1 | 2.5 ± 0.4 | .0101 | .0253 | .8679 | ||||
Naive CD4+ | 3.3 ± 1.8 | 7.5 ± 2.9 | 7.8 ± 0.7 | .0135 | .0172 | .8248 | ||||
Naive CD8+ | 0.4 ± 0.2 | 1.5 ± 0.7 | 1.8 ± 0.4 | .0111 | .0039 | .3274 | ||||
Host-type | ||||||||||
CD3+ | 14.3 ± 2.1 | 16.7 ± 3.5 | 17.4 ± 2.6 | .2372 | .1757 | .7111 | ||||
CD4+ | 8.1 ± 1.6 | 10.4 ± 2.0 | 11.6 ± 1.6 | .0631 | .0175 | .3117 | ||||
CD8+ | 5.4 ± 1.8 | 5.7 ± 1.7 | 4.8 ± 1.9 | .7743 | .6792 | .4554 | ||||
Naive CD4+ | 5.2 ± 1.9 | 6.9 ± 1.9 | 7.5 ± 1.3 | .1330 | .0881 | .6381 | ||||
Naive CD8+ | 0.6 ± 0.3 | 1.2 ± 0.4 | 1.6 ± 0.4 | .0333 | .0050 | .1677 |
. | Groups . | . | . | P . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|---|
. | 400 KTLS . | 1000 KTLS . | 5000 KTLS . | 400 vs 1000 . | 400 vs 5000 . | 1000 vs 5000 . | ||||
n | 4 | 7 | 4 | — | — | — | ||||
Total | ||||||||||
CD3+ | 19.6 ± 4.3 | 28.5 ± 5.6 | 30.4 ± 2.9 | .0115 | .0076 | .5369 | ||||
CD4+ | 11.3 ± 3.0 | 18.0 ± 3.4 | 18.9 ± 1.2 | .0032 | .0029 | .6087 | ||||
CD8+ | 6.3 ± 2.3 | 8.3 ± 2.2 | 7.3 ± 2.2 | .1814 | .5309 | .5028 | ||||
Naive CD4+ | 8.5 ± 3.5 | 14.4 ± 2.9 | 15.3 ± 1.3 | .0054 | .0049 | .6346 | ||||
Naive CD8+ | 0.9 ± 0.5 | 2.7 ± 1.0 | 3.4 ± 0.8 | .0088 | .0018 | .1941 | ||||
Donor-type | ||||||||||
CD3+ | 5.3 ± 2.3 | 11.8 ± 3.6 | 13.0 ± 0.6 | .0032 | .0023 | .5197 | ||||
CD4+ | 3.2 ± 1.6 | 7.5 ± 2.3 | 7.3 ± 0.4 | .0026 | .0079 | .8462 | ||||
CD8+ | 0.9 ± 0.5 | 2.6 ± 1.1 | 2.5 ± 0.4 | .0101 | .0253 | .8679 | ||||
Naive CD4+ | 3.3 ± 1.8 | 7.5 ± 2.9 | 7.8 ± 0.7 | .0135 | .0172 | .8248 | ||||
Naive CD8+ | 0.4 ± 0.2 | 1.5 ± 0.7 | 1.8 ± 0.4 | .0111 | .0039 | .3274 | ||||
Host-type | ||||||||||
CD3+ | 14.3 ± 2.1 | 16.7 ± 3.5 | 17.4 ± 2.6 | .2372 | .1757 | .7111 | ||||
CD4+ | 8.1 ± 1.6 | 10.4 ± 2.0 | 11.6 ± 1.6 | .0631 | .0175 | .3117 | ||||
CD8+ | 5.4 ± 1.8 | 5.7 ± 1.7 | 4.8 ± 1.9 | .7743 | .6792 | .4554 | ||||
Naive CD4+ | 5.2 ± 1.9 | 6.9 ± 1.9 | 7.5 ± 1.3 | .1330 | .0881 | .6381 | ||||
Naive CD8+ | 0.6 ± 0.3 | 1.2 ± 0.4 | 1.6 ± 0.4 | .0333 | .0050 | .1677 |
Lethally irradiated (10.5 Gy) CD45.2 mice received transplants of 3 different doses (400, 1000, and 5000) of KTLS cells that corresponded with T-cell-depleted bone marrow cell doses used in Table 2 (eg, 400 KTLS = 8 × 105 T-cell-depleted bone marrow cells) from the congenic CD45.1 mice. Mice were killed 42 days after transplantation. Spleen cells were prepared and analyzed by flow cytometry. The absolute counts were determined using Flow-Count fluorospheres. Naive T cells refer to T cells with CD62L +CD44− phenotype. All values represent total cell counts (× 106) per spleen (mean ± SD). The values of normal recipient-type mice (n = 5) are as follows: CD3+, 59 ± 7; CD4+, 28 ± 3; CD8+, 22 ± 4; naive CD4+, 26 ± 3; and naive CD8+, 15 ± 3. The values of normal donor-type mice (n = 5) are as follows: CD3+, 61 ± 10; CD4+, 31 ± 6; CD8+, 24 ± 4; naive CD4+, 28 ± 6; and naive CD8+, 17 ± 4. KTLS indicates c-Kit+Thy1.1lowLin−/lowSca-1+ hematopoietic stem cells; —indicates not applicable. CD45.2 mice indicates C57BL/6, CD45.2, Thy1.2 mice. CD45.1 mice indicates C57BL/Ka, CD45.1, Thy1.1 mice.