Table 4.

T-lymphocyte recovery in spleen after congenic purified hematopoietic stem cell transplantation (day + 42)



Groups

P

400 KTLS
1000 KTLS
5000 KTLS
400 vs 1000
400 vs 5000
1000 vs 5000
n   4   7   4   —   —   —  
Total       
CD3+  19.6 ± 4.3   28.5 ± 5.6   30.4 ± 2.9   .0115   .0076   .5369  
CD4+  11.3 ± 3.0   18.0 ± 3.4   18.9 ± 1.2   .0032   .0029   .6087  
CD8+  6.3 ± 2.3   8.3 ± 2.2   7.3 ± 2.2   .1814   .5309   .5028  
Naive CD4+  8.5 ± 3.5   14.4 ± 2.9   15.3 ± 1.3   .0054   .0049   .6346  
Naive CD8+  0.9 ± 0.5   2.7 ± 1.0   3.4 ± 0.8   .0088   .0018   .1941  
Donor-type       
CD3+  5.3 ± 2.3   11.8 ± 3.6   13.0 ± 0.6   .0032   .0023   .5197  
CD4+  3.2 ± 1.6   7.5 ± 2.3   7.3 ± 0.4   .0026   .0079   .8462  
CD8+  0.9 ± 0.5   2.6 ± 1.1   2.5 ± 0.4   .0101   .0253   .8679  
Naive CD4+  3.3 ± 1.8   7.5 ± 2.9   7.8 ± 0.7   .0135   .0172   .8248  
Naive CD8+  0.4 ± 0.2   1.5 ± 0.7   1.8 ± 0.4   .0111   .0039   .3274  
Host-type       
CD3+  14.3 ± 2.1   16.7 ± 3.5   17.4 ± 2.6   .2372   .1757   .7111  
CD4+  8.1 ± 1.6   10.4 ± 2.0   11.6 ± 1.6   .0631   .0175   .3117  
CD8+  5.4 ± 1.8   5.7 ± 1.7   4.8 ± 1.9   .7743   .6792   .4554  
Naive CD4+  5.2 ± 1.9   6.9 ± 1.9   7.5 ± 1.3   .1330   .0881   .6381  
Naive CD8+
 
0.6 ± 0.3
 
1.2 ± 0.4
 
1.6 ± 0.4
 
.0333
 
.0050
 
.1677
 


Groups

P

400 KTLS
1000 KTLS
5000 KTLS
400 vs 1000
400 vs 5000
1000 vs 5000
n   4   7   4   —   —   —  
Total       
CD3+  19.6 ± 4.3   28.5 ± 5.6   30.4 ± 2.9   .0115   .0076   .5369  
CD4+  11.3 ± 3.0   18.0 ± 3.4   18.9 ± 1.2   .0032   .0029   .6087  
CD8+  6.3 ± 2.3   8.3 ± 2.2   7.3 ± 2.2   .1814   .5309   .5028  
Naive CD4+  8.5 ± 3.5   14.4 ± 2.9   15.3 ± 1.3   .0054   .0049   .6346  
Naive CD8+  0.9 ± 0.5   2.7 ± 1.0   3.4 ± 0.8   .0088   .0018   .1941  
Donor-type       
CD3+  5.3 ± 2.3   11.8 ± 3.6   13.0 ± 0.6   .0032   .0023   .5197  
CD4+  3.2 ± 1.6   7.5 ± 2.3   7.3 ± 0.4   .0026   .0079   .8462  
CD8+  0.9 ± 0.5   2.6 ± 1.1   2.5 ± 0.4   .0101   .0253   .8679  
Naive CD4+  3.3 ± 1.8   7.5 ± 2.9   7.8 ± 0.7   .0135   .0172   .8248  
Naive CD8+  0.4 ± 0.2   1.5 ± 0.7   1.8 ± 0.4   .0111   .0039   .3274  
Host-type       
CD3+  14.3 ± 2.1   16.7 ± 3.5   17.4 ± 2.6   .2372   .1757   .7111  
CD4+  8.1 ± 1.6   10.4 ± 2.0   11.6 ± 1.6   .0631   .0175   .3117  
CD8+  5.4 ± 1.8   5.7 ± 1.7   4.8 ± 1.9   .7743   .6792   .4554  
Naive CD4+  5.2 ± 1.9   6.9 ± 1.9   7.5 ± 1.3   .1330   .0881   .6381  
Naive CD8+
 
0.6 ± 0.3
 
1.2 ± 0.4
 
1.6 ± 0.4
 
.0333
 
.0050
 
.1677
 

Lethally irradiated (10.5 Gy) CD45.2 mice received transplants of 3 different doses (400, 1000, and 5000) of KTLS cells that corresponded with T-cell-depleted bone marrow cell doses used in Table 2 (eg, 400 KTLS = 8 × 105 T-cell-depleted bone marrow cells) from the congenic CD45.1 mice. Mice were killed 42 days after transplantation. Spleen cells were prepared and analyzed by flow cytometry. The absolute counts were determined using Flow-Count fluorospheres. Naive T cells refer to T cells with CD62L +CD44 phenotype. All values represent total cell counts (× 106) per spleen (mean ± SD). The values of normal recipient-type mice (n = 5) are as follows: CD3+, 59 ± 7; CD4+, 28 ± 3; CD8+, 22 ± 4; naive CD4+, 26 ± 3; and naive CD8+, 15 ± 3. The values of normal donor-type mice (n = 5) are as follows: CD3+, 61 ± 10; CD4+, 31 ± 6; CD8+, 24 ± 4; naive CD4+, 28 ± 6; and naive CD8+, 17 ± 4. KTLS indicates c-Kit+Thy1.1lowLin−/lowSca-1+ hematopoietic stem cells; —indicates not applicable. CD45.2 mice indicates C57BL/6, CD45.2, Thy1.2 mice. CD45.1 mice indicates C57BL/Ka, CD45.1, Thy1.1 mice.

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