Table 3. Immune recovery in peripheral... | American Society of Hematology

Table 3.

Immune recovery in peripheral blood after congenic purified hematopoietic stem cell transplantation

	Total					Donor-type
	CD4⁺	CD8⁺	B220⁺	CD4⁺	CD8⁺	CD4⁺	CD8⁺
Before transplantation	773 ± 147	379 ± 69	2912 ± 714	—	—	—	—
Day + 28
400 KTLS	429 ± 98^*	265 ± 79	77 ± 60‡	2 ± 1†	1 ± 1†	427 ± 98^*	264 ± 78
1000 KTLS	548 ± 100	292 ± 101	465 ± 267	10 ± 6	5 ± 3	538 ± 97	287 ± 99
5000 KTLS	653 ± 213	316 ± 134	1481 ± 464	65 ± 71	23 ± 20	588 ± 176	292 ± 125
Day + 35
400 KTLS	633 ± 202†	383 ± 122	438 ± 364‡	48 ± 31‡	13 ± 8‡	431 ± 98†	278 ± 83
1000 KTLS	866 ± 143	412 ± 135	1201 ± 482	152 ± 80	45 ± 26	714 ± 114	367 ± 112
5000 KTLS	1028 ± 214	458 ± 137	2897 ± 540	366 ± 127	115 ± 38	662 ± 149	342 ± 135
Day + 42
400 KTLS	1130 ± 198†	575 ± 135	1556 ± 1408‡	401 ± 150‡	113 ± 44‡	729 ± 185	462 ± 155
1000 KTLS	1345 ± 211	628 ± 118	3056 ± 839	645 ± 151	208 ± 56	700 ± 123	420 ± 111
5000 KTLS	1563 ± 349	700 ± 184	4651 ± 845	937 ± 263	320 ± 101	626 ± 102	379 ± 89
Day + 56
400 KTLS	1486 ± 640	724 ± 328	2571 ± 2575^*	929 ± 464†	304 ± 169†	558 ± 219	420 ± 191
1000 KTLS	1371 ± 282	641 ± 83	2543 ± 1317	857 ± 187	317 ± 71	514 ± 159	324 ± 57
5000 KTLS	1816 ± 353	819 ± 158	4973 ± 1015	1387 ± 272	503 ± 103	429 ± 90	315 ± 97
Day + 70
400 KTLS	2109 ± 619^*	983 ± 302†	5721 ± 4035^*	1561 ± 486†	547 ± 185†	548 ± 174	436 ± 153†
1000 KTLS	1597 ± 507	680 ± 154	4316 ± 2137	1189 ± 434	413 ± 139	408 ± 113	267 ± 28
5000 KTLS	2476 ± 521	1111 ± 234	8148 ± 1873	2048 ± 441	802 ± 172	428 ± 84	309 ± 87

	Total					Donor-type
	CD4⁺	CD8⁺	B220⁺	CD4⁺	CD8⁺	CD4⁺	CD8⁺
Before transplantation	773 ± 147	379 ± 69	2912 ± 714	—	—	—	—
Day + 28
400 KTLS	429 ± 98^*	265 ± 79	77 ± 60‡	2 ± 1†	1 ± 1†	427 ± 98^*	264 ± 78
1000 KTLS	548 ± 100	292 ± 101	465 ± 267	10 ± 6	5 ± 3	538 ± 97	287 ± 99
5000 KTLS	653 ± 213	316 ± 134	1481 ± 464	65 ± 71	23 ± 20	588 ± 176	292 ± 125
Day + 35
400 KTLS	633 ± 202†	383 ± 122	438 ± 364‡	48 ± 31‡	13 ± 8‡	431 ± 98†	278 ± 83
1000 KTLS	866 ± 143	412 ± 135	1201 ± 482	152 ± 80	45 ± 26	714 ± 114	367 ± 112
5000 KTLS	1028 ± 214	458 ± 137	2897 ± 540	366 ± 127	115 ± 38	662 ± 149	342 ± 135
Day + 42
400 KTLS	1130 ± 198†	575 ± 135	1556 ± 1408‡	401 ± 150‡	113 ± 44‡	729 ± 185	462 ± 155
1000 KTLS	1345 ± 211	628 ± 118	3056 ± 839	645 ± 151	208 ± 56	700 ± 123	420 ± 111
5000 KTLS	1563 ± 349	700 ± 184	4651 ± 845	937 ± 263	320 ± 101	626 ± 102	379 ± 89
Day + 56
400 KTLS	1486 ± 640	724 ± 328	2571 ± 2575^*	929 ± 464†	304 ± 169†	558 ± 219	420 ± 191
1000 KTLS	1371 ± 282	641 ± 83	2543 ± 1317	857 ± 187	317 ± 71	514 ± 159	324 ± 57
5000 KTLS	1816 ± 353	819 ± 158	4973 ± 1015	1387 ± 272	503 ± 103	429 ± 90	315 ± 97
Day + 70
400 KTLS	2109 ± 619^*	983 ± 302†	5721 ± 4035^*	1561 ± 486†	547 ± 185†	548 ± 174	436 ± 153†
1000 KTLS	1597 ± 507	680 ± 154	4316 ± 2137	1189 ± 434	413 ± 139	408 ± 113	267 ± 28
5000 KTLS	2476 ± 521	1111 ± 234	8148 ± 1873	2048 ± 441	802 ± 172	428 ± 84	309 ± 87

Lethally irradiated (10.5 Gy) CD45.2 mice received transplants of 3 different doses (400, 1000, and 5000) of KTLS cells that corresponded with T-cell-depleted bone marrow cell doses used in Table 2 (eg, 400 KTLS = 8 × 10⁵ T-cell-depleted bone marrow cells) from the congenic CD45.1 mice. Peripheral blood was harvested at various time points after stem cell transplantation. The blood samples were then stained and analyzed by flow cytometry. The absolute counts were determined using Flow-Count fluorospheres. Data represent absolute count (mean ± SD/μL blood) in peripheral blood from 4 to 15 animals per group per time point. All B cells were donor origin (Figure 2). KTLS indicates c-Kit⁺Thy1.1^lowLin^−/lowSca-1⁺ hematopoietic stem cells; —, not done. CD45.2 mice indicates C57BL/6, CD45.2, Thy1.2 mice. CD45.1 mice indicates C57BL/Ka, CD45.1, Thy1.1 mice.

P < .05, in any one of the following comparisons: 400 KTLS versus 1000 KTLS, 400 KTLS versus 5000 KTLS, and 1000 KTLS versus 5000 KTLS.

†

P < .05, any 2 of the following comparisons: 400 KTLS versus 1000 KTLS, 400 KTLS versus 5000 KTLS, and 1000 KTLS versus 5000 KTLS.

‡

P < .05, all of the following comparisons: 400 KTLS versus 1000 KTLS, 400 KTLS versus 5000 KTLS, and 1000 KTLS versus 5000 KTLS.

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