Table 2. Immune recovery in peripheral... | American Society of Hematology

Table 2.

Immune recovery in peripheral blood after congenic T-cell-depleted bone marrow transplantation

	Total					Donor-type
	CD4⁺	CD8⁺	B220⁺	CD4⁺	CD8⁺	CD4⁺	CD8⁺
Before
transplantation	773 ± 147	379 ± 69	2912 ± 714	—	—	—	—
Day + 28
8 × 10⁵	476 ± 165	216 ± 63	190 ± 192‡	29 ± 36†	12 ± 16†	447 ± 182	204 ± 76
4 × 10⁶	526 ± 75	190 ± 47	702 ± 151	48 ± 59	13 ± 14	478 ± 96	177 ± 53
1 × 10⁷	565 ± 116	212 ± 54	1641 ± 476	201 ± 96	59 ± 30	364 ± 54	153 ± 34
Day + 35
8 × 10⁵	781 ± 220	371 ± 116^*	1112 ± 878†	158 ± 99†	40 ± 32†	623 ± 240†	331 ± 137†
4 × 10⁶	824 ± 171	278 ± 42	2093 ± 683	223 ± 122	57 ± 37	601 ± 136	221 ± 44
1 × 10⁷	973 ± 250	340 ± 67	2787 ± 885	568 ± 188	167 ± 51	405 ± 95	173 ± 23
Day + 42
8 × 10⁵	1037 ± 296†	526 ± 256	1544 ± 1220‡	446 ± 140†	132 ± 39†	592 ± 224^*	395 ± 240†
4 × 10⁶	1039 ± 334	387 ± 102	2752 ± 974	532 ± 267	156 ± 80	507 ± 120	230 ± 57
1 × 10⁷	1408 ± 289	505 ± 112	4627 ± 1554	1010 ± 255	306 ± 82	398 ± 70	199 ± 41
Day + 56
8 × 10⁵	1619 ± 557	727 ± 264	3246 ± 2993^*	1109 ± 446^*	389 ± 186^*	510 ± 195†	339 ± 148^*
4 × 10⁶	1744 ± 411	697 ± 126	4604 ± 1598	1236 ± 400	428 ± 128	508 ± 124	269 ± 55
1 × 10⁷	1944 ± 403	769 ± 170	6273 ± 2401	1600 ± 375	564 ± 131	344 ± 47	204 ± 47
Day + 70
8 × 10⁵	1571 ± 687^*	778 ± 350	3742 ± 3436^*	1214 ± 565†	475 ± 246†	357 ± 184	303 ± 166
4 × 10⁶	1606 ± 237	721 ± 76	5403 ± 1564	1249 ± 253	477 ± 108	358 ± 96	245 ± 67
1 × 10⁷	2059 ± 361	936 ± 180	7765 ± 2145	1811 ± 357	730 ± 139	249 ± 35	207 ± 53

	Total					Donor-type
	CD4⁺	CD8⁺	B220⁺	CD4⁺	CD8⁺	CD4⁺	CD8⁺
Before
transplantation	773 ± 147	379 ± 69	2912 ± 714	—	—	—	—
Day + 28
8 × 10⁵	476 ± 165	216 ± 63	190 ± 192‡	29 ± 36†	12 ± 16†	447 ± 182	204 ± 76
4 × 10⁶	526 ± 75	190 ± 47	702 ± 151	48 ± 59	13 ± 14	478 ± 96	177 ± 53
1 × 10⁷	565 ± 116	212 ± 54	1641 ± 476	201 ± 96	59 ± 30	364 ± 54	153 ± 34
Day + 35
8 × 10⁵	781 ± 220	371 ± 116^*	1112 ± 878†	158 ± 99†	40 ± 32†	623 ± 240†	331 ± 137†
4 × 10⁶	824 ± 171	278 ± 42	2093 ± 683	223 ± 122	57 ± 37	601 ± 136	221 ± 44
1 × 10⁷	973 ± 250	340 ± 67	2787 ± 885	568 ± 188	167 ± 51	405 ± 95	173 ± 23
Day + 42
8 × 10⁵	1037 ± 296†	526 ± 256	1544 ± 1220‡	446 ± 140†	132 ± 39†	592 ± 224^*	395 ± 240†
4 × 10⁶	1039 ± 334	387 ± 102	2752 ± 974	532 ± 267	156 ± 80	507 ± 120	230 ± 57
1 × 10⁷	1408 ± 289	505 ± 112	4627 ± 1554	1010 ± 255	306 ± 82	398 ± 70	199 ± 41
Day + 56
8 × 10⁵	1619 ± 557	727 ± 264	3246 ± 2993^*	1109 ± 446^*	389 ± 186^*	510 ± 195†	339 ± 148^*
4 × 10⁶	1744 ± 411	697 ± 126	4604 ± 1598	1236 ± 400	428 ± 128	508 ± 124	269 ± 55
1 × 10⁷	1944 ± 403	769 ± 170	6273 ± 2401	1600 ± 375	564 ± 131	344 ± 47	204 ± 47
Day + 70
8 × 10⁵	1571 ± 687^*	778 ± 350	3742 ± 3436^*	1214 ± 565†	475 ± 246†	357 ± 184	303 ± 166
4 × 10⁶	1606 ± 237	721 ± 76	5403 ± 1564	1249 ± 253	477 ± 108	358 ± 96	245 ± 67
1 × 10⁷	2059 ± 361	936 ± 180	7765 ± 2145	1811 ± 357	730 ± 139	249 ± 35	207 ± 53

Lethally irradiated (10.5 Gy) CD45.2 mice received transplants of 3 different doses (8 × 10⁵, 4 × 10⁶, and 1 × 10⁷) of T-cell-depleted bone marrow cells from the congenic CD45.1 mice. Peripheral blood was harvested at various time points after transplantation. The blood samples were then stained and analyzed by flow cytometry. The absolute counts were determined using Flow-Count fluorospheres. Data represent absolute count (mean ± SD/μL blood) in peripheral blood from 8 to 10 animals per group per time point. All B cells were donor origin (Figure 2). CD45.2 mice indicate C57BL/Ka, CD45.2, Thy1.2 mice. CD45.1 mice indicate C57BL/Ka, CD45.1, Thy1.1 mice.—indicates not applicable.

P < .05, in any one of the following comparisons: 8 × 10⁵ versus 4 × 10⁶, 8 × 10⁵ versus 1 × 10⁷, and 4 × 10⁶ versus 1 × 10⁷.

†

P < .05, any 2 of the following comparisons: 8 × 10⁵ versus 4 × 10⁶, 8 × 10⁵ versus 1 × 10⁷, and 4 × 10⁶ versus 1 × 10⁷.

‡

P < .05, all of the following comparisons: 8 × 10⁵ versus 4 × 10⁶, 8 × 10⁵ versus 1 × 10⁷, and 4 × 10⁶ versus 1 × 10⁷.

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