Immune recovery in spleen after allogeneic T-cell-depleted bone marrow transplantation
. | Day + 14 . | . | . | Day + 28 . | . | . | Day + 42 . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Phenotypes . | 1 × 107 . | 5 × 107 . | P . | 1 × 107 . | 5 × 107 . | P . | 1 × 107 . | 5 × 107 . | P . | ||||||
| n | 5 | 5 | — | 5 | 5 | — | 5 | 5 | — | ||||||
| Total spleen cells | 140 ± 38 | 296 ± 78 | .0083 | 207 ± 22 | 261 ± 50 | .0591 | 215 ± 38 | 250 ± 74 | .3676 | ||||||
| CD3+ | 9 ± 2 | 18 ± 5 | .0133 | 39 ± 10 | 65 ± 22 | .0660 | 60 ± 8 | 68 ± 17 | .4176 | ||||||
| CD4+ | 7 ± 2 | 15 ± 5 | .0159 | 33 ± 8 | 55 ± 20 | .0764 | 49 ± 6 | 55 ± 13 | .4305 | ||||||
| CD8+ | 2 ± 0.8 | 2 ± 0.6 | .3444 | 6 ± 2 | 10 ± 3 | .0448 | 9 ± 5 | 13 ± 4 | .1774 | ||||||
| CD4+CD62L+ | 2 ± 0.4 | 3 ± 0.9 | .0627 | 15 ± 5 | 24 ± 6 | .0373 | 34 ± 8 | 32 ± 17 | .8334 | ||||||
| CD8+CD62L+ | 0.2 ± 0.0 | 0.4 ± 0.1 | .0349 | 3 ± 1 | 5 ± 0.3 | .0559 | 9 ± 2 | 12 ± 3 | .2335 | ||||||
| B220+ | 77 ± 31 | 222 ± 69 | .0062 | 137 ± 15 | 178 ± 37 | .0750 | 110 ± 17 | 159 ± 55 | .1358 | ||||||
. | Day + 14 . | . | . | Day + 28 . | . | . | Day + 42 . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Phenotypes . | 1 × 107 . | 5 × 107 . | P . | 1 × 107 . | 5 × 107 . | P . | 1 × 107 . | 5 × 107 . | P . | ||||||
| n | 5 | 5 | — | 5 | 5 | — | 5 | 5 | — | ||||||
| Total spleen cells | 140 ± 38 | 296 ± 78 | .0083 | 207 ± 22 | 261 ± 50 | .0591 | 215 ± 38 | 250 ± 74 | .3676 | ||||||
| CD3+ | 9 ± 2 | 18 ± 5 | .0133 | 39 ± 10 | 65 ± 22 | .0660 | 60 ± 8 | 68 ± 17 | .4176 | ||||||
| CD4+ | 7 ± 2 | 15 ± 5 | .0159 | 33 ± 8 | 55 ± 20 | .0764 | 49 ± 6 | 55 ± 13 | .4305 | ||||||
| CD8+ | 2 ± 0.8 | 2 ± 0.6 | .3444 | 6 ± 2 | 10 ± 3 | .0448 | 9 ± 5 | 13 ± 4 | .1774 | ||||||
| CD4+CD62L+ | 2 ± 0.4 | 3 ± 0.9 | .0627 | 15 ± 5 | 24 ± 6 | .0373 | 34 ± 8 | 32 ± 17 | .8334 | ||||||
| CD8+CD62L+ | 0.2 ± 0.0 | 0.4 ± 0.1 | .0349 | 3 ± 1 | 5 ± 0.3 | .0559 | 9 ± 2 | 12 ± 3 | .2335 | ||||||
| B220+ | 77 ± 31 | 222 ± 69 | .0062 | 137 ± 15 | 178 ± 37 | .0750 | 110 ± 17 | 159 ± 55 | .1358 | ||||||
Lethally irradiated BALB/c mice (H2d) received transplants of 2 different doses (1 × 107 and 5 × 107) of T-cell-depleted bone marrow cells from the CD45.2 mice (H2b). Mice were killed at different time points after transplantation. Spleen cells were prepared and analyzed by flow cytometry. This is a representative of 3 similar experiments. Similar results were also obtained in peripheral blood (data not shown). All values represent total cell counts (× 106) per spleen (mean ± SD). The values of normal BALB/c mice (n = 20) are as follows: total spleen cells, 252 ± 55; CD3+, 133 ± 26; CD4+, 113 ± 24; CD8+, 20 ± 2; and B220+, 122 ± 25. CD45.2 mice indicate CD57BL/6, CD45.2, Thy1.2 mice.—indicates not applicable.