HSCs in the bone marrow of adult FVB-mdr-1a/1b−/− mice show a normal SP phenotype but a reduced ability to efflux Rhodamine-123
Genotype, phenotype*(% of total†), and no. of cells per mouse . | Positive mice/total . | HSC frequency‡(upper and lower limits) . |
|---|---|---|
| +/+ | ||
| Rho−/lo (0.05 ± 0.01) | 1/870 (1/520-1/1 400) | |
| 300 | 2/7 | |
| 100 | 0/14 | |
| 30 | 2/11 | |
| Rho+ (0.03 ± 0.01) | < 1/7 200¶ (< 1/2 700-1/19 000) | |
| 1000 | 0/6 | |
| 300 | 0/5 | |
| 90 | 0/2 | |
| −/− | ||
| Rho−/lo (0.09 ± 0.01) | 1/62 (1/44-1/87) | |
| 300 | 3/3 | |
| 100 | 6/7 | |
| 30 | 3/9 | |
| 10 | 0/2 | |
| Rho+ (0.06 ± 0.01) | 1/100 (1/73-1/150) | |
| 700 | 3/3 | |
| 200 | 8/8 | |
| 70 | 1/6 |
Genotype, phenotype*(% of total†), and no. of cells per mouse . | Positive mice/total . | HSC frequency‡(upper and lower limits) . |
|---|---|---|
| +/+ | ||
| Rho−/lo (0.05 ± 0.01) | 1/870 (1/520-1/1 400) | |
| 300 | 2/7 | |
| 100 | 0/14 | |
| 30 | 2/11 | |
| Rho+ (0.03 ± 0.01) | < 1/7 200¶ (< 1/2 700-1/19 000) | |
| 1000 | 0/6 | |
| 300 | 0/5 | |
| 90 | 0/2 | |
| −/− | ||
| Rho−/lo (0.09 ± 0.01) | 1/62 (1/44-1/87) | |
| 300 | 3/3 | |
| 100 | 6/7 | |
| 30 | 3/9 | |
| 10 | 0/2 | |
| Rho+ (0.06 ± 0.01) | 1/100 (1/73-1/150) | |
| 700 | 3/3 | |
| 200 | 8/8 | |
| 70 | 1/6 |
Sort gates are as defined in Figure 2.
Values represent the mean ± SEM from 3 experiments.
HSC frequencies were calculated with data obtained 4 months after transplantation. Values shown in parentheses are the upper and lower limits defined by ± SEM. Levels of PB cells derived from the test transplants in positive recipients at the time of analysis varied from 9% to 85%.
Calculated assuming one mouse given the maximum number of Rho+ cells tested had been positive for donor cells.