HSC frequency and distribution in different subsets of lin− bone marrow cells from adult mice given 5-FU 2 days previously
Phenotype (% of total*and no. cells per mouse . | No. positive mice/total no. mice . | HSC frequency†(upper and lower limits) . | % of HSCs in fraction‡ . |
|---|---|---|---|
| Lin− SP Rho− (0.11 ± 0.04) | 1/29 (1/18-1/48) | 56 | |
| 60 | 3/3 | ||
| 30 | 2/4 | ||
| Lin− SP Rho+ (0.14 ± 0.05) | 1/59 (1/41-1/85) | 35 | |
| 90 | 2/3 | ||
| 30 | 4/8 | ||
| 10 | 2/15 | ||
| Lin− non-SP Rho+ (2.0 ± 0.3) | < 1/5300 (1/2000-1/14 200) | 6 | |
| 2000 | 0/2 | ||
| 400 | 0/6 | ||
| Lin− non-SP Rho+ (2.9 ± 0.2) | < 1/16 000¶ (< 1/6000-< 1/44 000) | < 3¶ | |
| 3500 | 0/3 | ||
| 800 | 0/5 |
Phenotype (% of total*and no. cells per mouse . | No. positive mice/total no. mice . | HSC frequency†(upper and lower limits) . | % of HSCs in fraction‡ . |
|---|---|---|---|
| Lin− SP Rho− (0.11 ± 0.04) | 1/29 (1/18-1/48) | 56 | |
| 60 | 3/3 | ||
| 30 | 2/4 | ||
| Lin− SP Rho+ (0.14 ± 0.05) | 1/59 (1/41-1/85) | 35 | |
| 90 | 2/3 | ||
| 30 | 4/8 | ||
| 10 | 2/15 | ||
| Lin− non-SP Rho+ (2.0 ± 0.3) | < 1/5300 (1/2000-1/14 200) | 6 | |
| 2000 | 0/2 | ||
| 400 | 0/6 | ||
| Lin− non-SP Rho+ (2.9 ± 0.2) | < 1/16 000¶ (< 1/6000-< 1/44 000) | < 3¶ | |
| 3500 | 0/3 | ||
| 800 | 0/5 |
Values represent the mean ± SEM from 3 experiments.
HSC frequencies were calculated with data obtained 4 months after transplantation. Values shown in parentheses are the upper and lower limits defined by ± SEM. Levels of PB cells derived from the test transplants in the positive recipients at the time of analysis varied from 1.5% to 88%.
Calculated as described in Table 1.
Calculated assuming one mouse given the maximum number of cells tested had been positive for donor cells.