HSCs generated from non-SP fetal liver HSCs in mice that received transplants have a SP phenotype
Cells injected into primary mice . | Repopulation of primary mice . | . | Repopulation of secondary mice . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Phenotype (no. cells per mouse) . | Time after transplantation, mos. . | % donor WBCs . | Cells/mouse* . | No. positive mice/total no. . | FL-derived HSCs/1° BM† . | |||
| Lin− non-SP (25 000) | 11 | 85 | ||||||
| 21% of 1° BM SP | 3/3 | > 17‡ | ||||||
| 7% of 1° BM SP | 3/3 | (> 10-> 33) | ||||||
| 21% of 1° BM non-SP | 3/3 | 17 | ||||||
| 7% of 1° BM non-SP | 2/3 | (10-33) | ||||||
| Lin− non-SP CD45+ (4000) | 8 | 88 | ||||||
| 21% of 1° BM SP | 1/1 | > 17‡ | ||||||
| 7% of 1° BM SP | 3/3 | (> 8-> 33) | ||||||
| 21% of 1° BM non-SP | 2/3 | 6 | ||||||
| 7% of 1° BM non-SP | 1/2 | (3-11) | ||||||
Cells injected into primary mice . | Repopulation of primary mice . | . | Repopulation of secondary mice . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Phenotype (no. cells per mouse) . | Time after transplantation, mos. . | % donor WBCs . | Cells/mouse* . | No. positive mice/total no. . | FL-derived HSCs/1° BM† . | |||
| Lin− non-SP (25 000) | 11 | 85 | ||||||
| 21% of 1° BM SP | 3/3 | > 17‡ | ||||||
| 7% of 1° BM SP | 3/3 | (> 10-> 33) | ||||||
| 21% of 1° BM non-SP | 3/3 | 17 | ||||||
| 7% of 1° BM non-SP | 2/3 | (10-33) | ||||||
| Lin− non-SP CD45+ (4000) | 8 | 88 | ||||||
| 21% of 1° BM SP | 1/1 | > 17‡ | ||||||
| 7% of 1° BM SP | 3/3 | (> 8-> 33) | ||||||
| 21% of 1° BM non-SP | 2/3 | 6 | ||||||
| 7% of 1° BM non-SP | 1/2 | (3-11) | ||||||
The SP and non-SP fractions of bone marrow (BM) cells from primary (1°) recipients of previously transplanted fetal liver (FL) cells were isolated, and matched dilutions of cells from equivalent proportions (21% and 7%) of the contents of 2 tibiae and 2 femurs were injected into a total of 12 secondary recipients per experiment, 3 mice per dilution per subset.
The fetal liver—derived HSC content of each fraction of primary bone marrow cells (in 2 tibiae and 2 femurs) was calculated by multiplying the frequency of HSCs in that fraction by the number of cells present in that fraction. Levels of PB cells with the Ly5 phenotype of the original fetal liver cells that were found in the secondary mice 6 months after transplantation varied from 1.3% to 81%.
Calculated assuming one mouse given the minimum number of cells tested had been negative for donor cells.