HSC frequency and distribution in different subsets of juvenile murine bone marrow cells
Phenotype (% of total*) and no. cells per mouse . | No. positive mice/no. total . | HSC frequency†(upper and lower limits) . | % of HSCs in fraction‡ . |
|---|---|---|---|
| SP (0.04 ± 0.01) | 1/630 (1/440-1/900) | 1 | |
| 500 | 4/8 | ||
| 150 | 2/11 | ||
| 50 | 2/14 | ||
| Non-SP (99.97 ± 0.01) | 1/22 000 (1/17 000-1/29 000) | 99 | |
| 100 000 | 5/5 | ||
| 30 000 | 14/15 | ||
| 10 000 | 1/15 | ||
| Lin−SP Rho− (0.0015) | 1/26 (1/13-1/53) | 9 | |
| 10 | 2/5 | ||
| 7 | 0/1 | ||
| 6 | 0/1 | ||
| Lin−SP Rho+ (0.019) | 1/110 (1/60-1/210) | 27 | |
| 230 | 3/3 | ||
| 100 | 0/1 | ||
| Lin−non-SP Rho− (1.8) | < 1/5800¶ (< 1/2100-<1/16 000) | < 48¶ | |
| 3500 | 0/2 | ||
| 350 | 0/2 | ||
| Lin−non-SP Rho+ (18) | 1/170 000 (1/58 000-1/480 000) | 16 | |
| 48 000 | 0/2 | ||
| 16 000 | 0/1 | ||
| 4800 | 1/2 |
Phenotype (% of total*) and no. cells per mouse . | No. positive mice/no. total . | HSC frequency†(upper and lower limits) . | % of HSCs in fraction‡ . |
|---|---|---|---|
| SP (0.04 ± 0.01) | 1/630 (1/440-1/900) | 1 | |
| 500 | 4/8 | ||
| 150 | 2/11 | ||
| 50 | 2/14 | ||
| Non-SP (99.97 ± 0.01) | 1/22 000 (1/17 000-1/29 000) | 99 | |
| 100 000 | 5/5 | ||
| 30 000 | 14/15 | ||
| 10 000 | 1/15 | ||
| Lin−SP Rho− (0.0015) | 1/26 (1/13-1/53) | 9 | |
| 10 | 2/5 | ||
| 7 | 0/1 | ||
| 6 | 0/1 | ||
| Lin−SP Rho+ (0.019) | 1/110 (1/60-1/210) | 27 | |
| 230 | 3/3 | ||
| 100 | 0/1 | ||
| Lin−non-SP Rho− (1.8) | < 1/5800¶ (< 1/2100-<1/16 000) | < 48¶ | |
| 3500 | 0/2 | ||
| 350 | 0/2 | ||
| Lin−non-SP Rho+ (18) | 1/170 000 (1/58 000-1/480 000) | 16 | |
| 48 000 | 0/2 | ||
| 16 000 | 0/1 | ||
| 4800 | 1/2 |
Values represent the mean ± SEM from 4 experiments for assays of SP and non-SP cells, and from 2 other experiments for the 4 subpopulations of lin− cells.
HSC frequencies were calculated from data obtained 6 months after transplantation as described in the footnote to Table 1. Values shown in parentheses are the upper and lower limits defined by ± SEM. Levels of PB cells derived from the test transplants in the positive recipients at the time of analysis varied from 1% to 88%.
Calculated as described in Table 1. The discrepancy between the values obtained for the non-SP and lin− non-SP fractions is likely due to the fact that these were derived from separate experiments.
Calculated assuming one mouse was given the maximum number of cells tested had been positive for donor cells.