Splenic IL-4 ELISPOT assay in mice receiving BM transplant with anti-CD8, anti-CD40L, and 3 Gy TBI
. | Day 4 . | . | . | Day 8 . | . | . | Day 15 . | . | . | Day 36 . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | B6 . | B10.A . | 3rd party . | B6 . | B10.A . | 3rd party . | B6 . | B10.A . | 3rd party . | B6 . | B10.A . | 3rd party . | ||||||||
| nl B6 | 22 | 252 | 291 | 0 | 254 | 309 | 30 | 217 | 235 | 40 | 460 | 433 | ||||||||
| Conditioned control (no BMT) | 0 | 22 | 88 | 5 | 168 | 136 | 3 | 123 | 203 | 20 | 213 | 208 | ||||||||
| BMT with no MR1 | 202 | 188 | 205 | 0 | 563 | 78 | 0 | 416 | 240 | 0 | 411 | 211 | ||||||||
| Chimera 1 | 268 | 101 | 314 | 53 | 13 | 208 | 35 | 82 | 380 | 0 | 0 | 313 | ||||||||
| Chimera 2 | 143 | 0 | 295 | 25 | 78 | 240 | 15 | 0 | 160 | 0 | 0 | 193 | ||||||||
| Chimera 3 | 137 | 0 | 363 | 0 | 81 | 176 | ND | ND | ND | 3 | 23 | 225 | ||||||||
. | Day 4 . | . | . | Day 8 . | . | . | Day 15 . | . | . | Day 36 . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | B6 . | B10.A . | 3rd party . | B6 . | B10.A . | 3rd party . | B6 . | B10.A . | 3rd party . | B6 . | B10.A . | 3rd party . | ||||||||
| nl B6 | 22 | 252 | 291 | 0 | 254 | 309 | 30 | 217 | 235 | 40 | 460 | 433 | ||||||||
| Conditioned control (no BMT) | 0 | 22 | 88 | 5 | 168 | 136 | 3 | 123 | 203 | 20 | 213 | 208 | ||||||||
| BMT with no MR1 | 202 | 188 | 205 | 0 | 563 | 78 | 0 | 416 | 240 | 0 | 411 | 211 | ||||||||
| Chimera 1 | 268 | 101 | 314 | 53 | 13 | 208 | 35 | 82 | 380 | 0 | 0 | 313 | ||||||||
| Chimera 2 | 143 | 0 | 295 | 25 | 78 | 240 | 15 | 0 | 160 | 0 | 0 | 193 | ||||||||
| Chimera 3 | 137 | 0 | 363 | 0 | 81 | 176 | ND | ND | ND | 3 | 23 | 225 | ||||||||
Each value represents the mean number of spots (2 wells) obtained with 1 × 106 initial splenocytes. Chimeras received 3 Gy TBI, anti-CD8 mAb, MR1, and BMT; conditioned controls received TBI, anti-CD8, MR1; BMT with no MR1 received TBI, anti-CD8, and BMT. ND indicates not done.