Table 5. Cytogenetic data for 4 MDS... | American Society of Hematology

Table 5.

Cytogenetic data for 4 MDS samples

		%abnormal cells (abnormal/total nuclei or total colonies)
Patient no.		Initial sample^*					FACS-sorted human CD45/71⁺ cells from individual mice
Patient no.		FISH probe	Total cells	CFCs	LTC-ICs	Wk 3	Wk 5-7	Wk 8-12	Wk 15-23
1	8 centromere	64 (329/516)	100† (10/10)	100 (15/15)	28 (60/212)	29 (37/128)	9 (51/566)	2.7-1.6 (35/1309)-(12/772)
8	5q31	100† (23/23)	94 (17/18)	95 (18/19)	—	—	—	85 (128/150)
9	8 centromere	11 (56/500)	—	—	3; 11 (2/63;207/1845)	0.4; 9 (7, 18)‡ (10/2272; 119/1343)	—	—
11	8 centromere	8 (66/864)	0 (0/40)	0† (0/5)	1.5 (14/957)	1 (10/902)	—	0.6 (3/538)

		%abnormal cells (abnormal/total nuclei or total colonies)
Patient no.		Initial sample^*					FACS-sorted human CD45/71⁺ cells from individual mice
Patient no.		FISH probe	Total cells	CFCs	LTC-ICs	Wk 3	Wk 5-7	Wk 8-12	Wk 15-23
1	8 centromere	64 (329/516)	100† (10/10)	100 (15/15)	28 (60/212)	29 (37/128)	9 (51/566)	2.7-1.6 (35/1309)-(12/772)
8	5q31	100† (23/23)	94 (17/18)	95 (18/19)	—	—	—	85 (128/150)
9	8 centromere	11 (56/500)	—	—	3; 11 (2/63;207/1845)	0.4; 9 (7, 18)‡ (10/2272; 119/1343)	—	—
11	8 centromere	8 (66/864)	0 (0/40)	0† (0/5)	1.5 (14/957)	1 (10/902)	—	0.6 (3/538)

— indicates no data available.

Initial sample refers to the cells injected.

†

Analysis of G-banded metaphases.

‡

Numbers in parentheses are the % of cells with trisomy 8 in CD15/66b⁺ and CD19/20⁺ populations isolated as separate subsets from the BM of one mouse 7 weeks after transplantation.

View Large

This Feature Is Available To Subscribers Only