Distribution of human phenotypes in the BM of mice that received transplants
. | . | Wk 3 . | . | . | Wk 5-7 . | . | . | Wk 8-12 . | . | . | Wk 15-23 . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | Recipient genotype . | %E . | %GM . | %L . | %E . | %GM . | %L . | %E . | %GM . | %L . | %E . | %GM . | %L . | ||||||||
| 1 | β2m−/−-3/GM/SF | 0 | 96 | 0 | 0 | 94 | 0 | 0 | 93 | 0 | 0 | 94 | 0 | ||||||||
| 3 | β2m−/−-3/GM/SF | 0 | 91 ± 3 | 0 | — | — | — | — | — | — | — | — | — | ||||||||
| 7 | β2m−/−-3/GM/SF | 0 | 82 ± 10 | 0 | — | — | — | — | — | — | — | — | — | ||||||||
| 8 | β2m−/−-3/GM/SF | — | — | — | — | — | — | — | — | — | — | 90 | — | ||||||||
| 9 | β2m−/−-3/GM/SF | 0 | 92 | 0 | 0 | 95 | 0 | ||||||||||||||
| 10 | β2m−/−-3/GM/SF | 38 ± 33 | 52 ± 23 | 6 ± 5 | 0 | 92 ± 2 | 3 ± 1 | 0 | 75 ± 1 | 18 ± 2 | 0 | 70 ± 10 | 13 ± 7 | ||||||||
| 11 | β2m−/−-3/GM/SF | 3 | 92 | 0 | 0 | 92 | 0 | 0 | 92 | 0 | 0 | 92 | 0 | ||||||||
| Normal lin− BM | 61 ± 14 | 30 ± 9 | 0 | 1 ± 0.3 | 79 ± 3 | 10 ± 2 | 0 | 41 ± 2 | 62 ± 5 | 0 | 39 ± 16 | 52 ± 21 | |||||||||
| 2 | β2m−/− | 56 | 31 | 0 | — | — | — | —* | — | — | — | — | — | ||||||||
| 3 | β2m−/− | 0 | 96 ± 1 | 0 | 0 | 90 ± 4 | 0 | — | — | — | — | — | — | ||||||||
| 5 | β2m−/− | 52 ± 11 | 45 ± 3 | 0 | 0 | 91 ± 8 | 0 | —* | — | — | — | — | — | ||||||||
| 7 | β2m−/− | 0 | 86 ± 6 | 0 | 0 | 98 ± 1 | 0 | — | — | — | — | — | — | ||||||||
| 9 | β2m−/− | 4 | 90 | 0 | 0 | 78 | 20 | — | — | — | — | — | — | ||||||||
| 10 | β2m−/− | 0 | 80 ± 3 | 10 ± 3 | 0 | 60 ± 10 | 26 ± 9 | 0 | 30 ± 11 | 44 ± 14 | 0 | 11 ± 3 | 86 ± 10 | ||||||||
| Normal lin− BM | β2m−/− | 57 ± 5 | 20 ± 3 | 0 | 3 ± 1 | 57 ± 7 | 33 ± 7 | 0 | 9 ± 3 | 86 ± 4 | 0 | 27 ± 10 | 76 ± 11 | ||||||||
. | . | Wk 3 . | . | . | Wk 5-7 . | . | . | Wk 8-12 . | . | . | Wk 15-23 . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | Recipient genotype . | %E . | %GM . | %L . | %E . | %GM . | %L . | %E . | %GM . | %L . | %E . | %GM . | %L . | ||||||||
| 1 | β2m−/−-3/GM/SF | 0 | 96 | 0 | 0 | 94 | 0 | 0 | 93 | 0 | 0 | 94 | 0 | ||||||||
| 3 | β2m−/−-3/GM/SF | 0 | 91 ± 3 | 0 | — | — | — | — | — | — | — | — | — | ||||||||
| 7 | β2m−/−-3/GM/SF | 0 | 82 ± 10 | 0 | — | — | — | — | — | — | — | — | — | ||||||||
| 8 | β2m−/−-3/GM/SF | — | — | — | — | — | — | — | — | — | — | 90 | — | ||||||||
| 9 | β2m−/−-3/GM/SF | 0 | 92 | 0 | 0 | 95 | 0 | ||||||||||||||
| 10 | β2m−/−-3/GM/SF | 38 ± 33 | 52 ± 23 | 6 ± 5 | 0 | 92 ± 2 | 3 ± 1 | 0 | 75 ± 1 | 18 ± 2 | 0 | 70 ± 10 | 13 ± 7 | ||||||||
| 11 | β2m−/−-3/GM/SF | 3 | 92 | 0 | 0 | 92 | 0 | 0 | 92 | 0 | 0 | 92 | 0 | ||||||||
| Normal lin− BM | 61 ± 14 | 30 ± 9 | 0 | 1 ± 0.3 | 79 ± 3 | 10 ± 2 | 0 | 41 ± 2 | 62 ± 5 | 0 | 39 ± 16 | 52 ± 21 | |||||||||
| 2 | β2m−/− | 56 | 31 | 0 | — | — | — | —* | — | — | — | — | — | ||||||||
| 3 | β2m−/− | 0 | 96 ± 1 | 0 | 0 | 90 ± 4 | 0 | — | — | — | — | — | — | ||||||||
| 5 | β2m−/− | 52 ± 11 | 45 ± 3 | 0 | 0 | 91 ± 8 | 0 | —* | — | — | — | — | — | ||||||||
| 7 | β2m−/− | 0 | 86 ± 6 | 0 | 0 | 98 ± 1 | 0 | — | — | — | — | — | — | ||||||||
| 9 | β2m−/− | 4 | 90 | 0 | 0 | 78 | 20 | — | — | — | — | — | — | ||||||||
| 10 | β2m−/− | 0 | 80 ± 3 | 10 ± 3 | 0 | 60 ± 10 | 26 ± 9 | 0 | 30 ± 11 | 44 ± 14 | 0 | 11 ± 3 | 86 ± 10 | ||||||||
| Normal lin− BM | β2m−/− | 57 ± 5 | 20 ± 3 | 0 | 3 ± 1 | 57 ± 7 | 33 ± 7 | 0 | 9 ± 3 | 86 ± 4 | 0 | 27 ± 10 | 76 ± 11 | ||||||||
Values shown are the percentages of detectable glyA+ erythroid (E), CD33+ or CD15/66b+ granulopoietic/macrophage (GM) and CD34−CD19/20+ B-lymphoid (L) cells within the total human cell population measured in highly engrafted mice, where such measurements were possible. The percentage of cells in each subset is expressed as the mean ± SEM except for patients 1, 2, 8, 9, and 11, where data were from single mice. β2m−/−-3/GM/SF indicates NOD/SCID-β2m−/−-3/GM/SF; β2m−/−, NOD/SCID-β2m−/−; and —, no data available.
Experiments were terminated 5 weeks after transplantation.