Table 3.

Comparison of the absolute numbers of human cells in the BM of NOD/SCID-β2m−/− and NOD/SCID-β2m−/−-3/GM/SF mice at varying times after transplantation




No. of positive mice/no. of mice injected

Percentage of human CD45/71+ cells in BM aspirates (range delimited by ± SEM)*
Patient no.
Recipient genotype
3 wk
5-7 wk
8-12 wk
15-23 wk
1   β2m−/−-3/GM/SF   1/2   0.47   0.75   32, 15  42, 16 
1   β2m−/−  0/2   —   —   —   —  
3   β2m−/−-3/GM/SF   2/2   4.8, 0.6   0.25, 0.5   —   —  
3   β2m−/−  5/7   3.3 (1.2-9.2)   0.4 (0.3-0.5)   —   —  
7   β2m−/−-3/GM/SF   3/6   149, 1.4, 4   0.25, 0.3, 0.25   —   —  
7   β2m−/−  7/7   3 (1-11)   1 (0.5-2.3)   —   —  
8   β2m−/−-3/GM/SF   1/1   —   —   0.4   0.8  
8   β2m−/−  0/1   —   —   —   —  
9   β2m−/−-3/GM/SF   1/1   3   36   —   —  
9   β2m−/−  1/1   85   10   —   —  
10   β2m−/−-3/GM/SF   2/2   24, 2  387, 77  134, 16  138, 12 
10   β2m−/−  7/7   1 (0.7-1.5)   2.6 (1.6-4.4)   1.8 (1.1-2.9)   2.8 (1.5-5.2)  
11   β2m−/−-3/GM/SF   1/2   8   4   0.28   0.4-0.8  
11   β2m−/−  0/2   —   —   —   —  
Normal lin BM   β2m−/−-3/GM/SF   6/6   3.7 (2.3-6.1)   56 (37-83)  12 (8.5-16)   2.8 (1.2-6.4)  
Normal lin BM
 
β2m−/−
 
5/6
 
0.6 (0.3-1)
 
3.6 (1.9-6.9)
 
4.1 (2.2-7.6)
 
0.8 (0.5-1.4)
 



No. of positive mice/no. of mice injected

Percentage of human CD45/71+ cells in BM aspirates (range delimited by ± SEM)*
Patient no.
Recipient genotype
3 wk
5-7 wk
8-12 wk
15-23 wk
1   β2m−/−-3/GM/SF   1/2   0.47   0.75   32, 15  42, 16 
1   β2m−/−  0/2   —   —   —   —  
3   β2m−/−-3/GM/SF   2/2   4.8, 0.6   0.25, 0.5   —   —  
3   β2m−/−  5/7   3.3 (1.2-9.2)   0.4 (0.3-0.5)   —   —  
7   β2m−/−-3/GM/SF   3/6   149, 1.4, 4   0.25, 0.3, 0.25   —   —  
7   β2m−/−  7/7   3 (1-11)   1 (0.5-2.3)   —   —  
8   β2m−/−-3/GM/SF   1/1   —   —   0.4   0.8  
8   β2m−/−  0/1   —   —   —   —  
9   β2m−/−-3/GM/SF   1/1   3   36   —   —  
9   β2m−/−  1/1   85   10   —   —  
10   β2m−/−-3/GM/SF   2/2   24, 2  387, 77  134, 16  138, 12 
10   β2m−/−  7/7   1 (0.7-1.5)   2.6 (1.6-4.4)   1.8 (1.1-2.9)   2.8 (1.5-5.2)  
11   β2m−/−-3/GM/SF   1/2   8   4   0.28   0.4-0.8  
11   β2m−/−  0/2   —   —   —   —  
Normal lin BM   β2m−/−-3/GM/SF   6/6   3.7 (2.3-6.1)   56 (37-83)  12 (8.5-16)   2.8 (1.2-6.4)  
Normal lin BM
 
β2m−/−
 
5/6
 
0.6 (0.3-1)
 
3.6 (1.9-6.9)
 
4.1 (2.2-7.6)
 
0.8 (0.5-1.4)
 

β2m−/−-3/GM/SF indicates NOD/SCID-β2m−/−-3/GM/SF ; β2m−/−, NOD/SCID-β2m−/−; and —, no data available.

*

Values shown are the derived total numbers of human CD45/71+ cells (×105) assuming that 2 femurs and 2 tibiae represent 25% of all mouse BM cells and that the total cellularity of these 4 long bones was as measured in a large set of similarly treated mice.

Data are from a single mouse assessed at different times in the interval shown (the second mouse died 8 weeks after transplantation).

Values in the 2 different types of recipient are significantly different P <. 05.

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