Comparison of the percentages of human cells in the BM of NOD/SCID-β2m−/− and NOD/SCID-β2m−/−-3/GM/SF mice at varying times after transplantation
. | . | No. of positive mice/no. of mice injected . | Percentage of human CD45/71+ cells in BM aspirates (range delimited by ± SEM)* . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | Recipient genotype . | . | 3wk . | 5-7wk . | 8-12 wk . | 15-23 wk . | |||
| 1 | β2m−/−-3/GM/SF | 1/2 | 0.3 | 0.1 | 6.8, 5.1† | 7, 1.7† | |||
| 1 | β2m−/− | 0/2 | — | — | — | — | |||
| 3 | β2m−/−-3/GM/SF | 2/2 | 1.2, 0.2 | ≤ 0.1, 0.1 | — | — | |||
| 3 | β2m−/− | 5/7 | 1.0 (0.4-3.0) | 0.05 (0.03-0.07) | — | — | |||
| 7 | β2m−/−-3/GM/SF | 3/6 | 39, 0.4, 1.9 | ≤ 0.1, 0.1, ≤ 0.1 | — | — | |||
| 7 | β2m−/− | 7/7 | 1.1 (0.4-3.2) | 0.1 (0.04-0.3) | — | — | |||
| 8 | β2m−/−-3/GM/SF | 1/1 | — | — | 0.1 | 0.1 | |||
| 8 | β2m−/− | 0/1 | — | — | — | — | |||
| 9 | β2m−/−-3/GM/SF | 1/1 | 2.6 | 4.5 | — | — | |||
| 9 | β2m−/− | 1/1 | 43 | 1.5 | — | — | |||
| 10 | β2m−/−-3/GM/SF | 2/2 | 13, 1.0‡ | 48, 9.5‡ | 19, 2.5‡ | 20, 1.9‡ | |||
| 10 | β2m−/− | 7/7 | 0.4 (0.3-0.7) | 0.3 (0.2-0.7) | 0.2 (0.1-0.4) | 0.4 (0.2-0.8) | |||
| 11 | β2m−/−-3/GM/SF | 1/2 | 5.7 | 0.6 | ≤ 0.1 | ≤ 0.1-0.13 | |||
| 11 | β2m−/− | 0/2 | — | — | — | — | |||
| Normal lin− BM | β2m−/−-3/GM/SF | 6/6 | 1.7 (1.0-2.9) | 8.1 (5.7-12)‡ | 1.7 (1.5-2.4) | 0.4 (0.1-0.9) | |||
| Normal lin− BM | β2m−/− | 5/6 | 0.17 (0.1-0.4) | 0.5 (0.3-1.0) | 0.6 (0.3-1.2) | 0.1 (0.06-0.2) | |||
. | . | No. of positive mice/no. of mice injected . | Percentage of human CD45/71+ cells in BM aspirates (range delimited by ± SEM)* . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | Recipient genotype . | . | 3wk . | 5-7wk . | 8-12 wk . | 15-23 wk . | |||
| 1 | β2m−/−-3/GM/SF | 1/2 | 0.3 | 0.1 | 6.8, 5.1† | 7, 1.7† | |||
| 1 | β2m−/− | 0/2 | — | — | — | — | |||
| 3 | β2m−/−-3/GM/SF | 2/2 | 1.2, 0.2 | ≤ 0.1, 0.1 | — | — | |||
| 3 | β2m−/− | 5/7 | 1.0 (0.4-3.0) | 0.05 (0.03-0.07) | — | — | |||
| 7 | β2m−/−-3/GM/SF | 3/6 | 39, 0.4, 1.9 | ≤ 0.1, 0.1, ≤ 0.1 | — | — | |||
| 7 | β2m−/− | 7/7 | 1.1 (0.4-3.2) | 0.1 (0.04-0.3) | — | — | |||
| 8 | β2m−/−-3/GM/SF | 1/1 | — | — | 0.1 | 0.1 | |||
| 8 | β2m−/− | 0/1 | — | — | — | — | |||
| 9 | β2m−/−-3/GM/SF | 1/1 | 2.6 | 4.5 | — | — | |||
| 9 | β2m−/− | 1/1 | 43 | 1.5 | — | — | |||
| 10 | β2m−/−-3/GM/SF | 2/2 | 13, 1.0‡ | 48, 9.5‡ | 19, 2.5‡ | 20, 1.9‡ | |||
| 10 | β2m−/− | 7/7 | 0.4 (0.3-0.7) | 0.3 (0.2-0.7) | 0.2 (0.1-0.4) | 0.4 (0.2-0.8) | |||
| 11 | β2m−/−-3/GM/SF | 1/2 | 5.7 | 0.6 | ≤ 0.1 | ≤ 0.1-0.13 | |||
| 11 | β2m−/− | 0/2 | — | — | — | — | |||
| Normal lin− BM | β2m−/−-3/GM/SF | 6/6 | 1.7 (1.0-2.9) | 8.1 (5.7-12)‡ | 1.7 (1.5-2.4) | 0.4 (0.1-0.9) | |||
| Normal lin− BM | β2m−/− | 5/6 | 0.17 (0.1-0.4) | 0.5 (0.3-1.0) | 0.6 (0.3-1.2) | 0.1 (0.06-0.2) | |||
β2m−/−-3/GM/SF indicates NOD/SCID-β2m−/−-3/GM/SF ; β2m−/−, NOD/SCID-β2m−/−; and —, no data available.
Values shown are the percentages of human CD45/71+ cells in BM aspirates of individually analyzed mice or the geometric means when data for at least 3 mice were available.
Data are from a single mouse assessed at different times in the interval shown (the second mouse died 8 weeks after transplantation).
Values in the 2 different types of recipient are significantly different P <.05.