Hematopoietic colonies cloned from NOD/SCID mouse marrow
Human grafts . | % human cells . | Estimated number of human colonies* . |
|---|---|---|
| Primary CD34+ cells | 88 | 47 |
| Cytokines alone | 0 | 0 |
| Cytokines plus 5azaD | 12 | 9 |
| Cytokines plus TSA | 0 | 0 |
| Cytokines plus sequential 5azaD and TSA | 91 | 26 |
Human grafts . | % human cells . | Estimated number of human colonies* . |
|---|---|---|
| Primary CD34+ cells | 88 | 47 |
| Cytokines alone | 0 | 0 |
| Cytokines plus 5azaD | 12 | 9 |
| Cytokines plus TSA | 0 | 0 |
| Cytokines plus sequential 5azaD and TSA | 91 | 26 |
The table shows the number of colonies after plating marrow cells from mice that received human grafts 7 weeks earlier.
The estimated number of human colonies is determined by the percentage of human cells composing the pooled hematopoietic colonies from a group of mice that stained positively with mAbs against CD45/CD33 antibodies using FACS analysis multiplied by the total number of colonies enumerated