CFC potential of re-isolated CD34+CD90+ cells from cultured cells exposed to 5azaD and TSA
Cell subset . | CFU-GM . | BFU-E . | CFU-MIX . | Total . | Plating efficiency . |
|---|---|---|---|---|---|
| CD34+CD90+ | 56 | 7 | 3 | 66 | 13.2 |
| CD34+CD90- | 15 | 21 | 0 | 36 | 7.2 |
Cell subset . | CFU-GM . | BFU-E . | CFU-MIX . | Total . | Plating efficiency . |
|---|---|---|---|---|---|
| CD34+CD90+ | 56 | 7 | 3 | 66 | 13.2 |
| CD34+CD90- | 15 | 21 | 0 | 36 | 7.2 |
After 9 days of culture, cells were sorted into 2 subsets according to expression of CD90. Cells (500) were plated in 1 mL methylcellulose in duplicates and scored after 14 days. Each value represents the mean number of colonies scored from duplicate cultures.