Phenotypic analysis of CD34+CD90+ and CD34+CD90- cells treated with 5azaD and TSA
. | . | Following ex vivo culture for 9 days . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Starting cell subset . | Total starting CD34+CD90+ cell counts, × 104* . | Total cell counts, × 104 . | % CD34+CD90+ . | Number of CD34+CD90+, × 104 . | Fold expansion of CD34+CD90+ . | |||
| CD34+CD90+ | 1.5 (1.5) | 2.25 | 82.2 | 1.85 | 1.3 | |||
| CD34+CD90- | 3.5 (0) | 3.0 | 51.4 | 1.5 | NA | |||
| CD34+ | 11.0 (3.5) | 56.0 | 9.7 | 5.5 | 1.6 | |||
. | . | Following ex vivo culture for 9 days . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Starting cell subset . | Total starting CD34+CD90+ cell counts, × 104* . | Total cell counts, × 104 . | % CD34+CD90+ . | Number of CD34+CD90+, × 104 . | Fold expansion of CD34+CD90+ . | |||
| CD34+CD90+ | 1.5 (1.5) | 2.25 | 82.2 | 1.85 | 1.3 | |||
| CD34+CD90- | 3.5 (0) | 3.0 | 51.4 | 1.5 | NA | |||
| CD34+ | 11.0 (3.5) | 56.0 | 9.7 | 5.5 | 1.6 | |||
The table shows the effect of 5azaD and TSA on purified CD34+ marrow cells sorted using FACSVantage based on CD90 expression. This represents cells of a representative study. Similar results were obtained in an additional experiment. NA indicates not applicable.
Each number represents the total number of cells started at the beginning of the culture period. The number in parentheses represents the number of CD34+CD90+ cells at the start of the culture