Ability of CD34+ cells exposed to 5azaD and or TSA to form hematopoietic colonies
Condition . | % CD34+ cells . | CFU-GM* . | BFU-E* . | CFU-MIX* . | Total number of colonies/plate* . | Plating efficiencies*† . |
|---|---|---|---|---|---|---|
| Primary cells | 92.5 ± 2.5 | 52.0 ± 4.2 | 60.0 ± 1.8 | 10.0 ± 2.9 | 122.0 ± 4.9 | 12.2 ± 0.5 |
| Cytokines alone | 0.88 ± 0.06 | 7.8 ± 1.8 | 0.0 | 0.25 ± 0.25 | 8.0 ± 2.0 | 0.8 ± 0.2‡ |
| Cytokines plus 5azaD | 31 ± 9.4 | 73.0 ± 0.25 | 4 ± 1.5 | 20.8 ± 9.3 | 97.8 ± 11.1 | 9.8 ± 1.1 |
| Cytokines plus TSA | 4.3 ± 0.6 | 13.0 ± 6.6 | 0.0 | 1.3 ± 0.8 | 14.3 ± 8.1 | 1.4 ± 0.8 |
| Cytokines plus 5azaD/TSA | 50 ± 8.7 | 74.0 ± 4.1 | 2.0 ± 0.0 | 21.0 ± 6.0 | 97.0 ± 2.0 | 9.7 ± 0.2‡ |
| Cytokines plus 5-FU | 26 ± 2.4 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Condition . | % CD34+ cells . | CFU-GM* . | BFU-E* . | CFU-MIX* . | Total number of colonies/plate* . | Plating efficiencies*† . |
|---|---|---|---|---|---|---|
| Primary cells | 92.5 ± 2.5 | 52.0 ± 4.2 | 60.0 ± 1.8 | 10.0 ± 2.9 | 122.0 ± 4.9 | 12.2 ± 0.5 |
| Cytokines alone | 0.88 ± 0.06 | 7.8 ± 1.8 | 0.0 | 0.25 ± 0.25 | 8.0 ± 2.0 | 0.8 ± 0.2‡ |
| Cytokines plus 5azaD | 31 ± 9.4 | 73.0 ± 0.25 | 4 ± 1.5 | 20.8 ± 9.3 | 97.8 ± 11.1 | 9.8 ± 1.1 |
| Cytokines plus TSA | 4.3 ± 0.6 | 13.0 ± 6.6 | 0.0 | 1.3 ± 0.8 | 14.3 ± 8.1 | 1.4 ± 0.8 |
| Cytokines plus 5azaD/TSA | 50 ± 8.7 | 74.0 ± 4.1 | 2.0 ± 0.0 | 21.0 ± 6.0 | 97.0 ± 2.0 | 9.7 ± 0.2‡ |
| Cytokines plus 5-FU | 26 ± 2.4 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
The table shows the ability of CD34+ cells exposed to 5azaD and or TSA to form hematopoietic colonies. All values are presented as mean ± the standard error of the mean.
Each number represents the mean of 2 experiments
Plating efficiency is defined as total number of (hematopoietic colonies/total cells plated) × 100
There is a significant increase in the total number of colonies when sequential addition of 5azaD and TSA is compared with cytokines alone (P<.01)