Effects of drug treatment on numbers of CD34+ and CD34+CD90+ cells
Culture conditions . | Total mononuclear cell number, × 104 . | Absolute CD34+ number, × 104 . | Absolute CD34+CD90+ number, × 104 . | Fold expansion of CD34+CD90+ cells* . |
|---|---|---|---|---|
| Cytokines alone | 640.0 ± 17 | 6.0 ± 0.2 | 1.0 ± 0.4† | 0.6 ± 0.2 |
| Cytokines plus 5-FU | 0.3 ± 0.0 | 0.1 ± 0.0 | 0.0 | 0.0 |
| Cytokines plus 5azaD | 32.0 ± 5.0 | 10.0 ± 4.0 | 4.0 ± 1.7 | 1.7 ± 0.8 |
| Cytokines plus TSA | 470.0 ± 87.0 | 19.0 ± 1.0 | 4.0 ± 1.0 | 1.8 ± 0.4 |
| Cytokines plus 5azaD/TSA | 24.0 ± 4.0 | 12.0 ± 2.0 | 6.0 ± 2.0† | 2.5 ± 0.7† |
Culture conditions . | Total mononuclear cell number, × 104 . | Absolute CD34+ number, × 104 . | Absolute CD34+CD90+ number, × 104 . | Fold expansion of CD34+CD90+ cells* . |
|---|---|---|---|---|
| Cytokines alone | 640.0 ± 17 | 6.0 ± 0.2 | 1.0 ± 0.4† | 0.6 ± 0.2 |
| Cytokines plus 5-FU | 0.3 ± 0.0 | 0.1 ± 0.0 | 0.0 | 0.0 |
| Cytokines plus 5azaD | 32.0 ± 5.0 | 10.0 ± 4.0 | 4.0 ± 1.7 | 1.7 ± 0.8 |
| Cytokines plus TSA | 470.0 ± 87.0 | 19.0 ± 1.0 | 4.0 ± 1.0 | 1.8 ± 0.4 |
| Cytokines plus 5azaD/TSA | 24.0 ± 4.0 | 12.0 ± 2.0 | 6.0 ± 2.0† | 2.5 ± 0.7† |
The table shows the effect of drug treatment on numbers of hematopoietic progenitor cells. Each value represents the mean of 3 experiments ± the standard error of the mean.
Fold change is calculated as the change in CD34+CD90+ cell numbers of the treated cells as compared with the primary cell numbers
There was a significant increase in the number of CD34+CD90+ cells between the cells exposed to sequential 5azaD and TSA and the cells exposed to cytokines alone (P < .05, paired t test)