Effect of drug treatments on DNA methylation status
. | . | % methylation† . | . | |
|---|---|---|---|---|
| Cell population . | % methylation* . | γ . | ϵ . | |
| Primary CD34+ cells‡ | 90 | NA | NA | |
| Cytokines alone§ | 87 | 100 | 100 | |
| Cytokines plus 5azaD | 32 | NA | NA | |
| Cytokines plus 5azaD/TSA | 58 | 70 | 67 | |
. | . | % methylation† . | . | |
|---|---|---|---|---|
| Cell population . | % methylation* . | γ . | ϵ . | |
| Primary CD34+ cells‡ | 90 | NA | NA | |
| Cytokines alone§ | 87 | 100 | 100 | |
| Cytokines plus 5azaD | 32 | NA | NA | |
| Cytokines plus 5azaD/TSA | 58 | 70 | 67 | |
The table shows the effect of drug treatment on methylation status on position -256 of the γ-promotor region.
NA indicates not available.
Each value in this column represents the calculated methylation percent by densitometry by COBRA analysis of γ-globin gene
Bisulfite sequence analysis showing the percentage of methylated clones on 2 separate promoter sites of the ϵ- and γ-globin genes
Primary cells are the uncultured CD34+ cells selected from human marrow
CD34+ cells were cultured for 5 days in the cytokines IL-3, TPO, SCF, and FLT-3