Proteins identified by MALDI-ToF mass spectrometry
Spot . | Protein . | Molecular weight . | pl . | Normalized volume Lin-Sca+Kit+ . | Normalized volume Lin-Sca+Kit- . | Function . |
|---|---|---|---|---|---|---|
| 1 | Alcohol dehydrogenase | 42.7 | 6.12 | 0.204 | 0.067 | Metabolic |
| 2 | Actin | 41.7 | 5.29 | 1.501 | 1.697 | Cytoskeletal |
| 3 | Adseverin | 80.2 | 5.64 | 2.99 | 6.7 | Cytoskeletal |
| 4 | Serine protease inhibitor | 55.5 | 7.53 | 0.131 | 0.043 | Metabolic |
| 5 | Enolase | 47 | 6.35 | 0.631 | 0.269 | Metabolic |
| 6 | GAPDH | 47.6 | 8.14 | 1.313 | 0.807 | Metabolic |
| 7 | Pyruvate kinase | 50.7 | 7.42 | 0.39 | 0.169 | Metabolic |
| 8 | Transketolase | 67.6 | 7.23 | 0.389 | 0.205 | Metabolic |
| 9 | Gelsolin | 80.7 | 5.3 | 0.056 | 0.104 | Cytoskeletal |
Spot . | Protein . | Molecular weight . | pl . | Normalized volume Lin-Sca+Kit+ . | Normalized volume Lin-Sca+Kit- . | Function . |
|---|---|---|---|---|---|---|
| 1 | Alcohol dehydrogenase | 42.7 | 6.12 | 0.204 | 0.067 | Metabolic |
| 2 | Actin | 41.7 | 5.29 | 1.501 | 1.697 | Cytoskeletal |
| 3 | Adseverin | 80.2 | 5.64 | 2.99 | 6.7 | Cytoskeletal |
| 4 | Serine protease inhibitor | 55.5 | 7.53 | 0.131 | 0.043 | Metabolic |
| 5 | Enolase | 47 | 6.35 | 0.631 | 0.269 | Metabolic |
| 6 | GAPDH | 47.6 | 8.14 | 1.313 | 0.807 | Metabolic |
| 7 | Pyruvate kinase | 50.7 | 7.42 | 0.39 | 0.169 | Metabolic |
| 8 | Transketolase | 67.6 | 7.23 | 0.389 | 0.205 | Metabolic |
| 9 | Gelsolin | 80.7 | 5.3 | 0.056 | 0.104 | Cytoskeletal |
Identity of spots shown in Figure 4B and normalized volumes calculated relative to spots common to all gels. Values shown for spots 3 and 9 were calculated from the total amount detected in the “chain” of spots (ie, 7 for adseverin [spot 3] and 2 for gelsolin [spot 9]).
GAPDH indicates glyceraldehyde 3 phosphate.