Skin graft survival in reconstituted NOD/SCID-Tg mice
. | . | Individual graft survival, d . | . | |
|---|---|---|---|---|
| Group . | Transplant . | SLA-matched . | Third party . | |
| A (n = 5) | pBMT | > 120 | > 120 | |
| B (n = 4)* | pBMT; HuThy/Liv/HSC | > 120 (n = 3), > 40† | 14, 38, 55, > 40† | |
| C (n = 3)* | HuThy/Liv/HSC | 14, 38, > 120‡ | 38, 31, > 120‡ | |
. | . | Individual graft survival, d . | . | |
|---|---|---|---|---|
| Group . | Transplant . | SLA-matched . | Third party . | |
| A (n = 5) | pBMT | > 120 | > 120 | |
| B (n = 4)* | pBMT; HuThy/Liv/HSC | > 120 (n = 3), > 40† | 14, 38, 55, > 40† | |
| C (n = 3)* | HuThy/Liv/HSC | 14, 38, > 120‡ | 38, 31, > 120‡ | |
FCM analysis at week 10 after human tissue/cell transplantation confirmed that mice in groups B and C had comparable levels of human cell chimerism (HLA class 1+ cells in blood were 2%-37% for group B and 2%-31% for group C).
Died on day 40 with surviving grafts.
Histologic examination showed severe mononuclear cell infiltration in the graft removed on day 120.