Primers used for PCR assays
Molecule . | Forward primer . | Reverse primer . | Reference . |
|---|---|---|---|
| β-actin | CCTGGACTTCGAGCAAGAGA | ACTTGCGCTCAGGAGGAGCA | Karandikar et al39 |
| GAPDH | GGTATCGTGGAAGGACTCATGAC | ATGCCAGTGAGCTTCCCGTTCAGC | Bertolotto et al52 |
| IFN-γ | TTTAATGCAGGTCATTCAGATGTA | CACTTGGATGAGTTCATGTATTGC | Karandikar et al39 |
| IL-4 | GCCTCACAGAGCAGAAGACT | TCAGCTCGAACACTTTGAAT | Leroy et al53 |
| TNF-α | CGAGTCTGGGCAGGTCTACTTT | AAGCTGTAGGCCCCAGTGAGTT | Karandikar et al39 |
| TGF-β1 | GCCCTGGACACCAACTATTGC | GCTGCACTTGCAGGAGCGCAC | Karandikar et al39 |
| IL-2 | TACAACTGGAGCATTTACTG | GTTTCAGATCCCTTTAGTTC | Leroy et al53 |
| IL-10 | TGCCTGGTCCTCCTGACTGG | GCCTTGCTCTTGTTTTCACA | Leroy et al53 |
| CXCR3 | CACTGCCCTTCTCATTTGGAAACT | GCAAATATAGAGGTCTTGGGGAC | Nanki et al54 |
| CCR5 | TGCTACTCGGGAATCATAAAAACT | TTCTGAACTTCTCCCCGACAAA | Douglas et al55 |
| CCR4 | TTGGACTATGCCATCCAGGC | AATTCCCTCTGGAGAAACCC | van den Berg et al56 |
| IL 5 | CTGATAGCCAATGAGACTCT | TATTATCCACTCGGTGTTCA | Leroy et al53 |
Molecule . | Forward primer . | Reverse primer . | Reference . |
|---|---|---|---|
| β-actin | CCTGGACTTCGAGCAAGAGA | ACTTGCGCTCAGGAGGAGCA | Karandikar et al39 |
| GAPDH | GGTATCGTGGAAGGACTCATGAC | ATGCCAGTGAGCTTCCCGTTCAGC | Bertolotto et al52 |
| IFN-γ | TTTAATGCAGGTCATTCAGATGTA | CACTTGGATGAGTTCATGTATTGC | Karandikar et al39 |
| IL-4 | GCCTCACAGAGCAGAAGACT | TCAGCTCGAACACTTTGAAT | Leroy et al53 |
| TNF-α | CGAGTCTGGGCAGGTCTACTTT | AAGCTGTAGGCCCCAGTGAGTT | Karandikar et al39 |
| TGF-β1 | GCCCTGGACACCAACTATTGC | GCTGCACTTGCAGGAGCGCAC | Karandikar et al39 |
| IL-2 | TACAACTGGAGCATTTACTG | GTTTCAGATCCCTTTAGTTC | Leroy et al53 |
| IL-10 | TGCCTGGTCCTCCTGACTGG | GCCTTGCTCTTGTTTTCACA | Leroy et al53 |
| CXCR3 | CACTGCCCTTCTCATTTGGAAACT | GCAAATATAGAGGTCTTGGGGAC | Nanki et al54 |
| CCR5 | TGCTACTCGGGAATCATAAAAACT | TTCTGAACTTCTCCCCGACAAA | Douglas et al55 |
| CCR4 | TTGGACTATGCCATCCAGGC | AATTCCCTCTGGAGAAACCC | van den Berg et al56 |
| IL 5 | CTGATAGCCAATGAGACTCT | TATTATCCACTCGGTGTTCA | Leroy et al53 |
GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase; IFN-γ, interferon γ; TNF-α, tumor necrosis factor α; CXCR3, CXC chemokine receptor 3; and CCR5, CC chemokine receptor 5.