Purification of APCE from human plasma
Purification step . | Total activity,*FI × 10-3 . | Total protein, mg . | Specific activity, FI × 10-3/mg . | Purification, fold . | Yield, % . |
|---|---|---|---|---|---|
| Plasma, 2 L | 58 950 | 185 650 | 0.3 | 1 | 100.0 |
| Ammonium sulfate | 41 520 | 48 840 | 0.9 | 3 | 70.4 |
| Phenyl-Toyopearl | 27 964 | 320 | 87.4 | 291 | 47.4 |
| Q-Sepharose anion | 17 687 | 32 | 552.7 | 1 842 | 30.0 |
| T-gel thiophilic | 8 576 | 0.45 | 19 057.8 | 63 526 | 14.5 |
| Immunoaffinity | 4 738 | 0.09 | 52 644.4 | 175 481 | 8.0 |
Purification step . | Total activity,*FI × 10-3 . | Total protein, mg . | Specific activity, FI × 10-3/mg . | Purification, fold . | Yield, % . |
|---|---|---|---|---|---|
| Plasma, 2 L | 58 950 | 185 650 | 0.3 | 1 | 100.0 |
| Ammonium sulfate | 41 520 | 48 840 | 0.9 | 3 | 70.4 |
| Phenyl-Toyopearl | 27 964 | 320 | 87.4 | 291 | 47.4 |
| Q-Sepharose anion | 17 687 | 32 | 552.7 | 1 842 | 30.0 |
| T-gel thiophilic | 8 576 | 0.45 | 19 057.8 | 63 526 | 14.5 |
| Immunoaffinity | 4 738 | 0.09 | 52 644.4 | 175 481 | 8.0 |
Total activity is defined as total fluorescence intensity (FI) produced by APCE-catalyzed FRET peptide cleavage during 2-hour incubation at 22°C.