Analysis of AID-induced mutations in Iμ-Cμ intron
. | Stimulation conditions . | . | . | ||
---|---|---|---|---|---|
Splenocytes . | UNS . | LPS . | LPS-IL4 . | ||
Δ/Δ, no. (total no.) | |||||
3′Eμf/Cmr | 2/16 492 (19) | 3/17 360 (20) | 2/13 020 (15) | ||
VH7183/5′Eμb | 5/5 526 (9) | 6/5 526 (9) | 4/3 684 (6) | ||
+/+, no. (total no.) | |||||
Imf/5′Sμb | 4/9 702 (18) | 7/9 163 (17) | — | ||
3′Sμf/Cmr | 0/7 980 (20) | 2/8 379 (21) | — | ||
Δ/+, no. (total no.) | |||||
Imf/Cmr | 2/13 320 (20) | 2/12 654 (19) | — | ||
Imf/5′Sμb | 1/9 702 (18) | 3/10 241 (19) | — | ||
3′Sμf/Cmr | 0/8 379 (21) | 1/7 581 (19) | — | ||
H5′/H3′ | 2/13 000 (20) | 11/14 3000 (22) | — |
. | Stimulation conditions . | . | . | ||
---|---|---|---|---|---|
Splenocytes . | UNS . | LPS . | LPS-IL4 . | ||
Δ/Δ, no. (total no.) | |||||
3′Eμf/Cmr | 2/16 492 (19) | 3/17 360 (20) | 2/13 020 (15) | ||
VH7183/5′Eμb | 5/5 526 (9) | 6/5 526 (9) | 4/3 684 (6) | ||
+/+, no. (total no.) | |||||
Imf/5′Sμb | 4/9 702 (18) | 7/9 163 (17) | — | ||
3′Sμf/Cmr | 0/7 980 (20) | 2/8 379 (21) | — | ||
Δ/+, no. (total no.) | |||||
Imf/Cmr | 2/13 320 (20) | 2/12 654 (19) | — | ||
Imf/5′Sμb | 1/9 702 (18) | 3/10 241 (19) | — | ||
3′Sμf/Cmr | 0/8 379 (21) | 1/7 581 (19) | — | ||
H5′/H3′ | 2/13 000 (20) | 11/14 3000 (22) | — |
The number of mutations per total number of bases sequenced is indicated for each category of splenocytes under the corresponding stimulation conditions. The total number of independent clones sequenced is indicated in the parentheses. The maps (not to scale) in Figure 5 indicate the relative position of the primers used and the sequences examined on the alleles of the homozygous mutant (Δ/Δ) (A), the wt (+/+) (B), and the heterozygous (Δ/+) (C) splenocytes.
— indicates not applicable.