Table 1. Forward and reverse primers... | American Society of Hematology

Table 1.

Forward and reverse primers

Gene	Forward primers: 5′ to 3′	Reverse primers: 5′ to 3′
c-Myb	CCC TGA AGC GCC TAA TCA C	ATG GCG GAT CAA GTC TCC TC
Bcl-xL	TTG GAC AAT GGA CTG GTT GA	TGA GTG GAC GGT CAG TGT CT
SCL	ACC TCA CGG CAA GCT AAG TAA	ACG CCG TTG AGC AGG ACT A
EKLF	ACC ACC CTG GGA CAG TTT CT	GAA GGG TCC TCC GAT TTC AG
GATA-1	GGG AGC TGA CTT TCC CAG T	GTC TCC TCT GCC ACA AGG TC
GATA-2	CAA GGA TGG CGT CAA GTA CC	ACA GTA ATG GCG GCA CAA G
c-Kit	ACA GGA CCT CGG CTA ACA AA	AAC GAG TCA CGC TTC CTT CT
LMO2	CGG CCA TCG AAA GGA AGA	TTG ATC TTG GTC CAC TCG TAG A
β-Globin	CTG CAC TGT GAC AAG CTT CAT	CAT TGG CCA CTC CAA TCA C
β-Actin	GTG ACG AGG CCC AGA GCA AGA G	AGG GGC CGG ACT CAT CGT ACT
GFP	GTG GAT CGA TCT GAG AAC TT	GCG GAT CTT GAA GTT CAC
PU.1: P1	GGA TCT GAC CAA CCT GGA GC	—
PU.1: P2	GGA AGG GTT TTC CCT CAC C	—
PU.1: P3	AGC GAT GGA GAA AGC CAT AG	—
PU.1: P4	—	AGC ACC TCG CCG CTG AA

Gene	Forward primers: 5′ to 3′	Reverse primers: 5′ to 3′
c-Myb	CCC TGA AGC GCC TAA TCA C	ATG GCG GAT CAA GTC TCC TC
Bcl-xL	TTG GAC AAT GGA CTG GTT GA	TGA GTG GAC GGT CAG TGT CT
SCL	ACC TCA CGG CAA GCT AAG TAA	ACG CCG TTG AGC AGG ACT A
EKLF	ACC ACC CTG GGA CAG TTT CT	GAA GGG TCC TCC GAT TTC AG
GATA-1	GGG AGC TGA CTT TCC CAG T	GTC TCC TCT GCC ACA AGG TC
GATA-2	CAA GGA TGG CGT CAA GTA CC	ACA GTA ATG GCG GCA CAA G
c-Kit	ACA GGA CCT CGG CTA ACA AA	AAC GAG TCA CGC TTC CTT CT
LMO2	CGG CCA TCG AAA GGA AGA	TTG ATC TTG GTC CAC TCG TAG A
β-Globin	CTG CAC TGT GAC AAG CTT CAT	CAT TGG CCA CTC CAA TCA C
β-Actin	GTG ACG AGG CCC AGA GCA AGA G	AGG GGC CGG ACT CAT CGT ACT
GFP	GTG GAT CGA TCT GAG AAC TT	GCG GAT CTT GAA GTT CAC
PU.1: P1	GGA TCT GAC CAA CCT GGA GC	—
PU.1: P2	GGA AGG GTT TTC CCT CAC C	—
PU.1: P3	AGC GAT GGA GAA AGC CAT AG	—
PU.1: P4	—	AGC ACC TCG CCG CTG AA

— indicates that the same reverse primer was used to amplify the different PU.1 exons (see Figure 1A).