Sequence determination of clonal antigen receptor gene rearrangements in patient ALL-7 at time of diagnosis, relapse, and following tertiary engraftment in NOD/SCID mice
Rearrangement . | Sequence . |
|---|---|
| IGH | |
| Diagnosis | (VH4-39)CGAGAC..................CTCTAGGATATTGTAGTGGTGGTAGCTGCTAGG..................CTGGTTCGAC (JH5) |
| Relapse | (VH4-39)CGAGAC..................CTCTAGGATATTGTAGTGGTGGTAGCTGCTAGG..................CTGGTTCGAC (JH5) |
| NOD/SCID 3° | (VH4-39)CGAGAC..................CTCTAGGATATTGTAGTGGTGGTAGCTGCTAGG..................CTGGTTCGAC (JH5) |
| IGK-Kde | |
| Diagnosis | (Vκ3-20)CTCACCT.................................CC.................................AGCCCTAGT (Kde) |
| Relapse | (Vκ3-20)CTCACCT.................................CC.................................AGCCCTAGT (Kde) |
| NOD/SCID 3° | (Vκ3-20)CTCACCT.................................CC.................................AGCCCTAGT (Kde) |
| IGK-Kde | |
| Diagnosis | (Vκ3-20)AGCTCACCT...............................GCC...............................GGAGCCCTAGT (Kde) |
| Relapse | (Vκ3-20)AGCTCACCT...............................GCC...............................GGAGCCCTAGT (Kde) |
| NOD/SCID 3° | (Vκ3-20)AGCTCACCT...............................GCC...............................GGAGCCCTAGT (Kde) |
| IGK-intron Kde | |
| Diagnosis | (VκIntron)CAGCTTTCC...............................GG...............................GGGAGCCCTA (Kde) |
| Relapse | Clonal rearrangement lost |
| NOD/SCID 3° | Clonal rearrangement lost |
| TCRG | |
| Diagnosis | (VγGCGTGGG..................................TTGTC..................................GAATTATTAT (Jγ) |
| Relapse | (VγCTGGGAC...............................TCGCCGAGGGG...............................ATTATAAGAA (Jγ) |
| NOD/SCID 3° | (VγCTGGGAC...............................TCGCCGAGGGG...............................ATTATAAGAA (Jγ) |
Rearrangement . | Sequence . |
|---|---|
| IGH | |
| Diagnosis | (VH4-39)CGAGAC..................CTCTAGGATATTGTAGTGGTGGTAGCTGCTAGG..................CTGGTTCGAC (JH5) |
| Relapse | (VH4-39)CGAGAC..................CTCTAGGATATTGTAGTGGTGGTAGCTGCTAGG..................CTGGTTCGAC (JH5) |
| NOD/SCID 3° | (VH4-39)CGAGAC..................CTCTAGGATATTGTAGTGGTGGTAGCTGCTAGG..................CTGGTTCGAC (JH5) |
| IGK-Kde | |
| Diagnosis | (Vκ3-20)CTCACCT.................................CC.................................AGCCCTAGT (Kde) |
| Relapse | (Vκ3-20)CTCACCT.................................CC.................................AGCCCTAGT (Kde) |
| NOD/SCID 3° | (Vκ3-20)CTCACCT.................................CC.................................AGCCCTAGT (Kde) |
| IGK-Kde | |
| Diagnosis | (Vκ3-20)AGCTCACCT...............................GCC...............................GGAGCCCTAGT (Kde) |
| Relapse | (Vκ3-20)AGCTCACCT...............................GCC...............................GGAGCCCTAGT (Kde) |
| NOD/SCID 3° | (Vκ3-20)AGCTCACCT...............................GCC...............................GGAGCCCTAGT (Kde) |
| IGK-intron Kde | |
| Diagnosis | (VκIntron)CAGCTTTCC...............................GG...............................GGGAGCCCTA (Kde) |
| Relapse | Clonal rearrangement lost |
| NOD/SCID 3° | Clonal rearrangement lost |
| TCRG | |
| Diagnosis | (VγGCGTGGG..................................TTGTC..................................GAATTATTAT (Jγ) |
| Relapse | (VγCTGGGAC...............................TCGCCGAGGGG...............................ATTATAAGAA (Jγ) |
| NOD/SCID 3° | (VγCTGGGAC...............................TCGCCGAGGGG...............................ATTATAAGAA (Jγ) |
Randomly inserted bases are shown in bold. Underlined bases present in the IGH rearranged sequence indicate a D2-15 segment. The particular V and J segments present in each rearrangement are shown in parentheses. VκIntron refers to an intronic segment of kappa light chain between Jκ5 and Cκ. Kde refers to the kappa deleting element.