Comparison of CD2 expression determined by microarray, RT quantitative PCR, and flow cytometry
Patient code . | Microarray, expression values . | Flow cytometry, % CD2+ cells . | RT-PCR, CT values* . |
|---|---|---|---|
| 04018 | 922.63 | 98 | 19.15 |
| 28008 | 615.96 | 92 | 18.47 |
| 49004 | 437.42 | 99 | 19.97 |
| 20005 | 108.96 | 0 | 25.48 |
| 24006 | 763.291 | 98 | 18.18 |
| 19017 | 210.134 | 41 | 19.03 |
| 11002 | 252.323 | 40 | 20.62 |
| 16002 | 152.721 | 12 | 21.91 |
| 16007 | 1165.110 | 95 | 17.44 |
| 19014 | 112.067 | 2 | 24.62 |
| 28009 | 112.967 | 19 | 24.42 |
| 43015 | 147.197 | 4 | 22.29 |
| 65003 | 269.643 | 69 | 20.00 |
| 01003 | 116.687 | 1 | 24.00 |
| 19002 | 199.305 | 21 | 21.58 |
| 37001 | 375.106 | 96 | 18.77 |
| 44001 | 149.088 | 2 | 23.44 |
| 10005 | 266.717 | 96 | 18.37 |
Patient code . | Microarray, expression values . | Flow cytometry, % CD2+ cells . | RT-PCR, CT values* . |
|---|---|---|---|
| 04018 | 922.63 | 98 | 19.15 |
| 28008 | 615.96 | 92 | 18.47 |
| 49004 | 437.42 | 99 | 19.97 |
| 20005 | 108.96 | 0 | 25.48 |
| 24006 | 763.291 | 98 | 18.18 |
| 19017 | 210.134 | 41 | 19.03 |
| 11002 | 252.323 | 40 | 20.62 |
| 16002 | 152.721 | 12 | 21.91 |
| 16007 | 1165.110 | 95 | 17.44 |
| 19014 | 112.067 | 2 | 24.62 |
| 28009 | 112.967 | 19 | 24.42 |
| 43015 | 147.197 | 4 | 22.29 |
| 65003 | 269.643 | 69 | 20.00 |
| 01003 | 116.687 | 1 | 24.00 |
| 19002 | 199.305 | 21 | 21.58 |
| 37001 | 375.106 | 96 | 18.77 |
| 44001 | 149.088 | 2 | 23.44 |
| 10005 | 266.717 | 96 | 18.37 |
Patient codes refer to CEL files. Pearson correlation coefficients: microarray and flow cytometry, 0.76; microarray and RT-PCR, −0.70; and RT-PCR and flow cytometry, −0.89.
CT values are rounded at 2 decimals.