Quantitation of altered AHI-1 isoform expression in human leukemic cell lines from Northern blot analysis of total RNA
. | Relative transcript levels for different AHI-1 mRNA species . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Cells . | 6.5 kb . | 5.0 kb . | 4.2 kb . | 2.0 kb . | 1.2 kb . | ||||
| NBM | 1 | 1 | 1 | 1 | 1 | ||||
| U937 | 6 | — | 7 | — | — | ||||
| HL60 | 14 | — | 12 | — | — | ||||
| K562 | 18 | 21 | 35 | 19 | — | ||||
| THP-1 | 14 | — | 16 | — | — | ||||
| IM9 | 3 | — | 3 | — | — | ||||
| Daudi | 4 | — | 3 | — | — | ||||
| Raji | 12 | — | 25 | — | — | ||||
| Jurkat | 6 | — | 5 | — | — | ||||
| CCL119 | 12 | — | 14 | — | — | ||||
| MT-4 | 14 | — | 12 | — | — | ||||
| Hut102 | 46 | 34 | 40 | — | 15 | ||||
| Hut 78 | 49 | — | 52 | — | 16 | ||||
. | Relative transcript levels for different AHI-1 mRNA species . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Cells . | 6.5 kb . | 5.0 kb . | 4.2 kb . | 2.0 kb . | 1.2 kb . | ||||
| NBM | 1 | 1 | 1 | 1 | 1 | ||||
| U937 | 6 | — | 7 | — | — | ||||
| HL60 | 14 | — | 12 | — | — | ||||
| K562 | 18 | 21 | 35 | 19 | — | ||||
| THP-1 | 14 | — | 16 | — | — | ||||
| IM9 | 3 | — | 3 | — | — | ||||
| Daudi | 4 | — | 3 | — | — | ||||
| Raji | 12 | — | 25 | — | — | ||||
| Jurkat | 6 | — | 5 | — | — | ||||
| CCL119 | 12 | — | 14 | — | — | ||||
| MT-4 | 14 | — | 12 | — | — | ||||
| Hut102 | 46 | 34 | 40 | — | 15 | ||||
| Hut 78 | 49 | — | 52 | — | 16 | ||||
The levels of each type of AHI-1 transcript were quantified and normalized to actin transcript levels as described in “Patients, materials, and methods” and then the ratio normalized again to the corresponding value obtained for unfractionated normal bone marrow (NBM) cells.
— indicates no signal to be detected.