Effect of CD3/CD28 costimulation and mDC coculture on the CD8+ cell noncytotoxic anti-HIV response
Subject . | αCD3/CD28 . | mDC . |
|---|---|---|
| 1 | 87.5 | 68.4 |
| 2 | 68.1 | 74.3 |
| 3 | 14.3 | 54.1 |
| 4 | 25.8 | 32 |
| 5 | 4.6 | 28 |
| 6 | 4.6 | 55.8 |
| 7 | 27.4 | 61.7 |
| 8 | 13.0 | 56.5 |
Subject . | αCD3/CD28 . | mDC . |
|---|---|---|
| 1 | 87.5 | 68.4 |
| 2 | 68.1 | 74.3 |
| 3 | 14.3 | 54.1 |
| 4 | 25.8 | 32 |
| 5 | 4.6 | 28 |
| 6 | 4.6 | 55.8 |
| 7 | 27.4 | 61.7 |
| 8 | 13.0 | 56.5 |
Data indicate percent of suppression of HIV-1 replication in infected CD4+ cells by CD8+ cells at a 1:1 CD8+ cell/CD4+ cell input ratio. The CD8+ cells were treated with antibodies to CD3 and CD28 or cocultured with mature dendritic cells (mDCs) (see “Patients, materials, and methods” for details on procedures). Control-infected CD4+ cells showed substantial HIV-1 replication (reverse transcriptase activity at > 2 × 106 cpm/mL of culture fluid).