Effects of NG-monomethyl-L-arginine on zileuton-and hydroxyurea-mediated BFU-E colony growth and fetal hemoglobin production in erythroid progenitors
. | ZL+LNMMA, 100 . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
. | n . | CON . | ZL, 50 μM . | μM . | HU, 100 μM . | HU+LNMMA, 100 μM . | |||||
| BFU-E | 4 | 124.4 ± 1.3 | 24.3 ± 2.3 | 27.5 ± 2.3 | 8.2 ± 0.9 | 20.8 ± 1.7* | |||||
| Hb F, % | 4 | 1.8 ± 0.2 | 11.5 ± 0.6 | 9.4 ± 0.7 | 19.7 ± 0.6 | 14.8 ± 1.1* | |||||
. | ZL+LNMMA, 100 . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
. | n . | CON . | ZL, 50 μM . | μM . | HU, 100 μM . | HU+LNMMA, 100 μM . | |||||
| BFU-E | 4 | 124.4 ± 1.3 | 24.3 ± 2.3 | 27.5 ± 2.3 | 8.2 ± 0.9 | 20.8 ± 1.7* | |||||
| Hb F, % | 4 | 1.8 ± 0.2 | 11.5 ± 0.6 | 9.4 ± 0.7 | 19.7 ± 0.6 | 14.8 ± 1.1* | |||||
BFU-E indicates burst-forming unit-erythroid; Hb F, fetal hemoglobin; CON, control; ZL, zileuton; LNMMA, NG-monomethyl-L-arginine; and HU, hydroxyurea.
P < .01 compared with HU alone.