Effects of zileuton on BFU-E colony growth and fetal hemoglobin production in primary erythroid cultures
. | Zileuton concentrations, μM . | . | . | . | . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | 0 . | . | 25 . | . | 50 . | . | 75 . | . | 100 . | . | |||||||||
| . | BFU-E . | Hb F, % . | BFU-E . | Hb F, % . | BFU-E . | Hb F, % . | BFU-E . | Hb F, % . | BFU-E . | Hb F, % . | |||||||||
| 1 | 87 | 2 | 61 | 4 | 47 | 7 | 27 | 11 | 12 | 14 | |||||||||
| 2 | 135 | 7 | 91 | 11 | 72 | 14 | 43 | 18 | 7 | 21 | |||||||||
| 3 | 148 | 2 | 137 | 3 | 62 | 6 | 23 | 11 | 13 | 13 | |||||||||
| 4 | 93 | 3 | 86 | 5 | 66 | 7 | 34 | 9 | 21 | 16 | |||||||||
| Mean | 116 | 3.5 | 93.8 | 5.8 | 61.8 | 8.5 | 31.8 | 12.3 | 13.3 | 16.0 | |||||||||
| SEM | 15.2 | 1.2 | 15.8 | 1.8 | 5.3 | 1.8 | 4.4 | 2.0 | 2.9 | 1.8 | |||||||||
| P | — | — | > .05 | > .05 | < .01 | > .05 | < .01 | < .01 | < .01 | < .01 | |||||||||
. | Zileuton concentrations, μM . | . | . | . | . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | 0 . | . | 25 . | . | 50 . | . | 75 . | . | 100 . | . | |||||||||
| . | BFU-E . | Hb F, % . | BFU-E . | Hb F, % . | BFU-E . | Hb F, % . | BFU-E . | Hb F, % . | BFU-E . | Hb F, % . | |||||||||
| 1 | 87 | 2 | 61 | 4 | 47 | 7 | 27 | 11 | 12 | 14 | |||||||||
| 2 | 135 | 7 | 91 | 11 | 72 | 14 | 43 | 18 | 7 | 21 | |||||||||
| 3 | 148 | 2 | 137 | 3 | 62 | 6 | 23 | 11 | 13 | 13 | |||||||||
| 4 | 93 | 3 | 86 | 5 | 66 | 7 | 34 | 9 | 21 | 16 | |||||||||
| Mean | 116 | 3.5 | 93.8 | 5.8 | 61.8 | 8.5 | 31.8 | 12.3 | 13.3 | 16.0 | |||||||||
| SEM | 15.2 | 1.2 | 15.8 | 1.8 | 5.3 | 1.8 | 4.4 | 2.0 | 2.9 | 1.8 | |||||||||
| P | — | — | > .05 | > .05 | < .01 | > .05 | < .01 | < .01 | < .01 | < .01 | |||||||||
P value represent a comparison of BFU-E colonies and Hb F in the absence of zileuton (0 μM) versus 25 to 100 μM concentrations. BFU-E indicates burst-forming unit-erythroid; Hb F, fetal hemoglobin; and —, not applicable.