Regulation of Ig production by ISC by cytokines
. | IgM . | . | IgG . | . | IgA . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Stimulus . | ng/mL . | Fold increase . | ng/mL . | Fold increase . | ng/mL . | Fold increase . | |||
| Nil | 301 ± 35 | 1.0 | 21 ± 6 | 1.0 | 87 ± 16 | 1.0 | |||
| IL-6 | 778 ± 72 | 1.9 ± 0.3 | 31 ± 3 | 1.3 ± 0.2 | 204 ± 33 | 1.9 ± 0.3 | |||
| IL-10 | 801 ± 132 | 2.5 ± 0.6 | 48 ± 19 | 1.5 ± 0.2 | 144 ± 31 | 2.6 ± 0.5 | |||
. | IgM . | . | IgG . | . | IgA . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Stimulus . | ng/mL . | Fold increase . | ng/mL . | Fold increase . | ng/mL . | Fold increase . | |||
| Nil | 301 ± 35 | 1.0 | 21 ± 6 | 1.0 | 87 ± 16 | 1.0 | |||
| IL-6 | 778 ± 72 | 1.9 ± 0.3 | 31 ± 3 | 1.3 ± 0.2 | 204 ± 33 | 1.9 ± 0.3 | |||
| IL-10 | 801 ± 132 | 2.5 ± 0.6 | 48 ± 19 | 1.5 ± 0.2 | 144 ± 31 | 2.6 ± 0.5 | |||
CD38++CD20± B cells were isolated by cell sorting and cultured in duplicate (approximately 5 × 103 cells/200 μL) in medium only (Nil) or in the presence of IL-6 (10 ng/mL) or IL-10 (100 U/mL). After 5 days, the level of Ig in culture supernatants was determined using ELISA. Values for Ig secretion are the means ± SD of 1 experiment (representative of 6) performed using cells from different donor spleens. Values for fold increase represent the mean increase ± SEM in Ig secretion induced by IL-6 or IL-10 (n = 6) relative to cultures performed in media alone.