Exclusion of 6 FA patient cell lines from known complementation groups: cell fusion experiments
. | Complementation group . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell line . | A . | B . | C . | D1 . | E . | F . | G . | ||||||
| EUFA592 | 75 ± 13.2 | > 100 ± 0 | 30 ± 13.9 | 24.5 ± 4.9 | 76 ± 8.5 | 60 ± 0 | 90 ± 14.1 | ||||||
| N = 3 | N = 3 | N = 6 | N = 2 | N = 2 | N = 2 | N = 2 | |||||||
| EUFA816 | 23 ± 2.6 | 23 ± 10.0 | 21 ± 16.2 | 34 ± 2.1 | 18 ± 0 | Failed | 100 ± 0 | ||||||
| N = 3 | N = 3 | N = 3 | N = 2 | N = 1 | (4 ×) | N = 2 | |||||||
| EUFA961 | 57 ± 12.7 | 37 ± 7.1 | ND | 73 ± 11.1 | > 100 ± 0 | ND | ND | ||||||
| N = 4 | N = 2 | N = 6 | N = 4 | ||||||||||
| EUFA696 | 69 ± 10.6 | 83 ± 5.2 | 28 ± 0 | 40 ± 0 | 75 ± 23.0 | Failed | 58 ± 25.0 | ||||||
| N = 4 | N = 3 | N = 2 | N = 1 | N = 3 | (18 ×) | N = 4 | |||||||
| EUFA543 | 68 ± 8.7 | 44 ± 11.5 | Failed | 83 ± 24.7 | 68 ± 27.5 | Failed | 33 ± 6.4 | ||||||
| N = 4 | N = 3 | (4 ×) | N = 2 | N = 3 | (8 ×) | N = 3 | |||||||
| AG656 | 55 ± 12.9 | 58 ± 15.8 | 47 ± 11.5 | > 100 ± 0 | > 100 ± 0 | 36 ± 19.8 | 39 ± 27.6 | ||||||
| N = 3 | N = 3 | N = 3 | N = 2 | N = 2 | N = 2 | N = 3 | |||||||
. | Complementation group . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell line . | A . | B . | C . | D1 . | E . | F . | G . | ||||||
| EUFA592 | 75 ± 13.2 | > 100 ± 0 | 30 ± 13.9 | 24.5 ± 4.9 | 76 ± 8.5 | 60 ± 0 | 90 ± 14.1 | ||||||
| N = 3 | N = 3 | N = 6 | N = 2 | N = 2 | N = 2 | N = 2 | |||||||
| EUFA816 | 23 ± 2.6 | 23 ± 10.0 | 21 ± 16.2 | 34 ± 2.1 | 18 ± 0 | Failed | 100 ± 0 | ||||||
| N = 3 | N = 3 | N = 3 | N = 2 | N = 1 | (4 ×) | N = 2 | |||||||
| EUFA961 | 57 ± 12.7 | 37 ± 7.1 | ND | 73 ± 11.1 | > 100 ± 0 | ND | ND | ||||||
| N = 4 | N = 2 | N = 6 | N = 4 | ||||||||||
| EUFA696 | 69 ± 10.6 | 83 ± 5.2 | 28 ± 0 | 40 ± 0 | 75 ± 23.0 | Failed | 58 ± 25.0 | ||||||
| N = 4 | N = 3 | N = 2 | N = 1 | N = 3 | (18 ×) | N = 4 | |||||||
| EUFA543 | 68 ± 8.7 | 44 ± 11.5 | Failed | 83 ± 24.7 | 68 ± 27.5 | Failed | 33 ± 6.4 | ||||||
| N = 4 | N = 3 | (4 ×) | N = 2 | N = 3 | (8 ×) | N = 3 | |||||||
| AG656 | 55 ± 12.9 | 58 ± 15.8 | 47 ± 11.5 | > 100 ± 0 | > 100 ± 0 | 36 ± 19.8 | 39 ± 27.6 | ||||||
| N = 3 | N = 3 | N = 3 | N = 2 | N = 2 | N = 2 | N = 3 | |||||||
Data on hybrids generated from the indicated cell lines and reference cell lines A-G are summarized. Numbers are IC50 values for MMC ± SD. Data do not include group D2, since these fusions had exceedingly low success rates; a retroviral vector expressing FANCD2 was used instead (Table 2).
ND indicates no data available.