Effect of different hepcidin dose regimens on iron absorption in mice
Treatment . | n . | Mucosal retention, pmol/mg . | Mucosal transfer, pmol/mg . | Total mucosal uptake, pmol/mg . |
|---|---|---|---|---|
| Intraperitoneal injection* | ||||
| Control | 4 | 17.7 ± 3.2 | 30.4 ± 5.9 | 48.1 ± 5.6 |
| 50 μg hepcidin × 1 | 4 | 17.7 ± 2.4 | 20.4 ± 3.8 | 38.1 ± 6.1 |
| Intraperitoneal injection† | ||||
| Control | 4 | 25.4 ± 2.0 | 21.9 ± 3.4 | 47.4 ± 3.1 |
| 50 μg hepcidin × 1 | 4 | 19.1 ± 2.9 | 18.8 ± 1.6 | 37.8 ± 3.7 |
| Control | 4 | 21.2 ± 3.1 | 27.3 ± 3.3 | 48.5 ± 4.1 |
| 50 μg hepcidin × 2 | 4 | 13.2 ± 2.2 | 19.3 ± 1.9 | 32.5 ± 3.6‡ |
| Subcutaneous injection† | ||||
| Control | 4 | 20.4 ± 0.8 | 30.1 ± 2.6 | 50.6 ± 2.5 |
| 50 μg hepcidin × 1 | 6 | 16.6 ± 0.9‡ | 16.5 ± 0.6§ | 33.1 ± 1.2§ |
Treatment . | n . | Mucosal retention, pmol/mg . | Mucosal transfer, pmol/mg . | Total mucosal uptake, pmol/mg . |
|---|---|---|---|---|
| Intraperitoneal injection* | ||||
| Control | 4 | 17.7 ± 3.2 | 30.4 ± 5.9 | 48.1 ± 5.6 |
| 50 μg hepcidin × 1 | 4 | 17.7 ± 2.4 | 20.4 ± 3.8 | 38.1 ± 6.1 |
| Intraperitoneal injection† | ||||
| Control | 4 | 25.4 ± 2.0 | 21.9 ± 3.4 | 47.4 ± 3.1 |
| 50 μg hepcidin × 1 | 4 | 19.1 ± 2.9 | 18.8 ± 1.6 | 37.8 ± 3.7 |
| Control | 4 | 21.2 ± 3.1 | 27.3 ± 3.3 | 48.5 ± 4.1 |
| 50 μg hepcidin × 2 | 4 | 13.2 ± 2.2 | 19.3 ± 1.9 | 32.5 ± 3.6‡ |
| Subcutaneous injection† | ||||
| Control | 4 | 20.4 ± 0.8 | 30.1 ± 2.6 | 50.6 ± 2.5 |
| 50 μg hepcidin × 1 | 6 | 16.6 ± 0.9‡ | 16.5 ± 0.6§ | 33.1 ± 1.2§ |
Mice were injected with 0.15 M NaCl (controls) or the indicated dose of hepcidin dissolved in 0.15 M NaCl either once or twice with an 8-hour gap between the 2 injections.
Data are expressed as means ± SEM for the number of mice per group.
Iron absorption was studied 4 hours after injections.
Iron absorption was studied 24 hours after injections.
P < .05 compared with appropriate control.
P < .001 compared with appropriate control.