DHPLC temperature and gradient conditions for HAMP coding fragments, with a flow rate of 0.9 mL/min and buffer B increased at 2% per minute
Exon . | Temperature, °C . | Acetonitrile gradient, buffer B, % . | Retention time, min . |
|---|---|---|---|
| 1 | 64 | 53-59 | 4.1 |
| 2-3 | 60 | 58-64 | 4.1 |
| 2-3 | 64 | 56-62 | 4.1 |
Exon . | Temperature, °C . | Acetonitrile gradient, buffer B, % . | Retention time, min . |
|---|---|---|---|
| 1 | 64 | 53-59 | 4.1 |
| 2-3 | 60 | 58-64 | 4.1 |
| 2-3 | 64 | 56-62 | 4.1 |