Somatic mutations in S107 VH sequences cloned from class-switched B cells of VavP-Bcl2 transgenic mice
Parameter . | Productive sequences . | Nonproductive sequences . | P (productive vs nonproductive) . | P (targeted productive vs random)* . |
|---|---|---|---|---|
| Mutations | 94 | 63 | .93 | |
| Ratio of hot spot to nonhot spot | 41:53 | 25:38 | .62 | <.0001 |
| Ratio of R to S† | 58:36 | 52:11 | .0052 | |
| R (ratio of C to F)‡ | 26:32 | 19:33 | .38 | .0011 |
| Mutations (ratio of C to F) | 40:54 | 21:42 | .25 | .0005 |
| F (ratio of R to S)§ | 32:22∥ | 33:9∥ | .045 | |
| C (ratio R to S)§ | 26:14∥ | 19:2∥ | .032 |
Parameter . | Productive sequences . | Nonproductive sequences . | P (productive vs nonproductive) . | P (targeted productive vs random)* . |
|---|---|---|---|---|
| Mutations | 94 | 63 | .93 | |
| Ratio of hot spot to nonhot spot | 41:53 | 25:38 | .62 | <.0001 |
| Ratio of R to S† | 58:36 | 52:11 | .0052 | |
| R (ratio of C to F)‡ | 26:32 | 19:33 | .38 | .0011 |
| Mutations (ratio of C to F) | 40:54 | 21:42 | .25 | .0005 |
| F (ratio of R to S)§ | 32:22∥ | 33:9∥ | .045 | |
| C (ratio R to S)§ | 26:14∥ | 19:2∥ | .032 |
S107 sequences were cloned from the PCR product derived from sorted B220+ IgM− IgD− κ+ spleen cells pooled from 2 VavP-Bcl2 mice. For productive sequences, n = 14; for nonproductive sequences, n = 9.
Tests of whether mutations in productive sequences were significantly concentrated in the 36-base hot spots and 72-base C regions of the 300-base length analyzed
Replacement (R) vs silent (S) mutations
Replacement mutations in complementarity-determining (C) vs framework (F) regions
Replacement vs silent mutations in F and C regions, respectively
Ratios of replacement to silent mutations were not significantly different between C and F regions in productive (P = .57) or nonproductive (P = .24) sequences