Kinetic parameters of the interactions between immobilized TfR and soluble Tf or A24
Density of immobilized receptor and ligand . | kon, 105 M-1 × s-1 . | koff, 10-4 × s-1 . | K′d, nM* . |
|---|---|---|---|
| Low | |||
| Fe-Tf | 12.1 ± 1.70 | 11.9 ± 1.9 | 0.98 ± 0.21 |
| mAb A24 | 8.30 ± 0.64 | 22.3 ± 4.8 | 2.69 ± 0.62 |
| High | |||
| Fe-Tf | 3.04 ± 0.63 | 9.48 ± 2.24 | 3.12 ± 0.93 |
| mAb A24 | 3.82 ± 0.12 | 3.88 ± 0.91 | 1.02 ± 0.24 |
Density of immobilized receptor and ligand . | kon, 105 M-1 × s-1 . | koff, 10-4 × s-1 . | K′d, nM* . |
|---|---|---|---|
| Low | |||
| Fe-Tf | 12.1 ± 1.70 | 11.9 ± 1.9 | 0.98 ± 0.21 |
| mAb A24 | 8.30 ± 0.64 | 22.3 ± 4.8 | 2.69 ± 0.62 |
| High | |||
| Fe-Tf | 3.04 ± 0.63 | 9.48 ± 2.24 | 3.12 ± 0.93 |
| mAb A24 | 3.82 ± 0.12 | 3.88 ± 0.91 | 1.02 ± 0.24 |
The determination of kon, koff, and K′d from BIAcore experimental data is described in “Materials and methods.” The mean value and associated SE of 3 or more independent determinations is given.
K′d is the equilibrium dissociation constant measured at the heterogeneous interface between the liquid phase and the sensorchip surface. There is no simple relationship between this constant and the Kd that would be measured in a homogeneous solution.