List of differentially regulated proteins identified after platelet activation with TRAP (5 μM, 30 s)
Protein name . | AN . | Mass, kDa . | pI . | Gel* . | Fold† . | Protein function‡ . |
|---|---|---|---|---|---|---|
| PDZ and LIM domain protein 1 (CLP-36) | O00151 | 19308 | 6.10 | 6-11 | B | May act as an adapter that recruits proteins to the cytoskeleton. |
| RGS10 | O43665 | 19716 | 6.88 | 6-11 | B | Inhibits signal transduction by increasing the GTPase activity of G protein α-subunits. |
| Dok-2 | O60496 | 51806 | 5.36 | 4-7 | 2.19 | Adapter downstream of tyrosine kinases. |
| Coagulation factor XIII A chain (precursor) | P00488 | 87939 | 5.73 | 4-7 | B | Coagulation factor, activated by thrombin. |
| Triosephosphate isomerase | P00938 | 25397 | 6.51 | 6-11 | B | Plays an important role in several metabolic pathways. |
| β or γ actin | P02570 or P02571 | 45146 | 5.67 | 4-7 | B | Highly conserved proteins involved in various types of cell motility. |
| Fibrinogen α/α-E chain (precursor) | P02671 | 63758 | 7.40 | 6-11 | -2.55 | Coagulation factor, which is secreted by α-granules in platelets. |
| 61108 | 6.52 | 6-11 | 3.18 | |||
| Fibrinogen β chain (precursor) | P02675 | 52836 | 7.82 | 6-11 | -2.71 | Coagulation factor, which is secreted by α-granules in platelets. |
| Glyceraldehyde 3-phosphate dehydrogenase, liver | P04406 | 35221 | 8.56 | 6-11 | 3.06 | Second phase of glycolysis; first step. |
| HSP27 | P04792 | 26201 | 5.67 | 4-7 | T | Implicated in signaling events associated to platelet aggregation. |
| 26576 | 5.34 | 4-7 | B | |||
| Tropomyosin alpha 4 chain | P07226 | 29042 | 11.34 | 6-11 | B | Implicated in stabilizing cytoskeleton actin filaments. |
| Pleckstrin | P08567 | 42219 | 7.02 | 6-11 | -3.06 | Major PKC substrate of platelets. |
| 42636 | 6.15 | 4-7 | 2.15 | |||
| 42859 | 6.19 | 4-7 | -2.29 | |||
| 42636 | 6.34 | 4-7 | 2.09 | |||
| 43076 | 6.01 | 4-7 | T | |||
| 43076 | 6.32 | 4-7 | T | |||
| 42433 | 7.17 | 6-11 | -2.32 | |||
| 60-kDa heat shock protein, mitochondrial (precursor) | P10809 | 60580 | 5.22 | 4-7 | -2.98 | Implicated in mitochondrial protein import and macromolecular assembly. |
| 78-kDa glucose-regulated protein (precursor) | P11021 | 74103 | 5.02 | 4-7 | T | Facilitates the assembly of multimeric protein complexes inside the ER. |
| 73240 | 5.00 | 4-7 | T | |||
| Destrin | P18282 | 15816 | 6.28 | 4-7 | B | Actin-depolymerizing protein. |
| Myosin regulatory light chain 2 | P24844 | 19452 | 4.68 | 4-7 | -2.29 | Plays an important role in cytoskeletal regulation. |
| ERp29 (precursor) | P30040 | 27238 | 5.82 | 6-11 | 2.08 | Plays an important role in the processing of secretory proteins within the ER. |
| RKIP | P30086 | 20527 | 7.35 | 6-11 | B | Regulates Raf-1. |
| Mannose-6-phosphate isomerase | P34949 | 42747 | 5.46 | 4-7 | T | Glycosylation; early steps of mannosylation. |
| 14-3-3-γ | P35214 | 27834 | 4.74 | 4-7 | T | Inhibits PKC in platelets. |
| Transgelin 2 | P37802 | 20527 | 7.35 | 6-11 | B | Participates in cytoskeletal regulation pathways. |
| 21313 | 6.75 | 4-7 | 2.02 | |||
| α-Synuclein | P37840 | 18348 | 4.69 | 4-7 | -2.4 | Molecular chaperone. |
| Proteasome subunit β type 3 | P49720 | 21807 | 5.91 | 4-7 | -2.9 | Proteasome pathway. |
| Phenol-sulfating phenol sulfotransferase 1 | P50225 | 29941 | 5.93 | 4-7 | B | Catalyses the sulfate conjugation of catecholamines. |
| ILK-2 | P57043 | 50288 | 7.67 | 6-11 | 2.44 | Receptor-proximal protein kinase regulating integrin-mediated signal transduction |
| Autoantigen protein (fragment) | Q16081 | 91999 | 5.03 | 4-7 | T | Unknown |
| Ubiquitin-conjugating enzyme E2 N | Q16781 | 14522 | 5.72 | 4-7 | B | Catalyzes the covalent attachment of ubiquitin to other proteins. |
| 5′ Nucleotidase, deoxy (pyrimidine), cytosolic type C | Q8TCD5 | 24602 | 5.79 | 6-11 | 2 | Catalyzes the dephosphorylation of pyrimidine 5-prime monophosphates. |
| Hypothetical protein | Q96GF6 | 18726 | 5.79 | 4-7 | B | Possible phosphatase. Function unknown. |
| D-prohibitin | Q99623 | 31011 | 9.52 | 6-11 | B | Integral membrane protein, thought to regulate cation conductance. |
| RGS18 | Q9NS28 | 27711 | 6.94 | 6-11 | B | Inhibits signal transduction by increasing the GTPase activity of G protein α-subunits. |
| 27397 | 6.34 | 6-11 | 2.34 |
Protein name . | AN . | Mass, kDa . | pI . | Gel* . | Fold† . | Protein function‡ . |
|---|---|---|---|---|---|---|
| PDZ and LIM domain protein 1 (CLP-36) | O00151 | 19308 | 6.10 | 6-11 | B | May act as an adapter that recruits proteins to the cytoskeleton. |
| RGS10 | O43665 | 19716 | 6.88 | 6-11 | B | Inhibits signal transduction by increasing the GTPase activity of G protein α-subunits. |
| Dok-2 | O60496 | 51806 | 5.36 | 4-7 | 2.19 | Adapter downstream of tyrosine kinases. |
| Coagulation factor XIII A chain (precursor) | P00488 | 87939 | 5.73 | 4-7 | B | Coagulation factor, activated by thrombin. |
| Triosephosphate isomerase | P00938 | 25397 | 6.51 | 6-11 | B | Plays an important role in several metabolic pathways. |
| β or γ actin | P02570 or P02571 | 45146 | 5.67 | 4-7 | B | Highly conserved proteins involved in various types of cell motility. |
| Fibrinogen α/α-E chain (precursor) | P02671 | 63758 | 7.40 | 6-11 | -2.55 | Coagulation factor, which is secreted by α-granules in platelets. |
| 61108 | 6.52 | 6-11 | 3.18 | |||
| Fibrinogen β chain (precursor) | P02675 | 52836 | 7.82 | 6-11 | -2.71 | Coagulation factor, which is secreted by α-granules in platelets. |
| Glyceraldehyde 3-phosphate dehydrogenase, liver | P04406 | 35221 | 8.56 | 6-11 | 3.06 | Second phase of glycolysis; first step. |
| HSP27 | P04792 | 26201 | 5.67 | 4-7 | T | Implicated in signaling events associated to platelet aggregation. |
| 26576 | 5.34 | 4-7 | B | |||
| Tropomyosin alpha 4 chain | P07226 | 29042 | 11.34 | 6-11 | B | Implicated in stabilizing cytoskeleton actin filaments. |
| Pleckstrin | P08567 | 42219 | 7.02 | 6-11 | -3.06 | Major PKC substrate of platelets. |
| 42636 | 6.15 | 4-7 | 2.15 | |||
| 42859 | 6.19 | 4-7 | -2.29 | |||
| 42636 | 6.34 | 4-7 | 2.09 | |||
| 43076 | 6.01 | 4-7 | T | |||
| 43076 | 6.32 | 4-7 | T | |||
| 42433 | 7.17 | 6-11 | -2.32 | |||
| 60-kDa heat shock protein, mitochondrial (precursor) | P10809 | 60580 | 5.22 | 4-7 | -2.98 | Implicated in mitochondrial protein import and macromolecular assembly. |
| 78-kDa glucose-regulated protein (precursor) | P11021 | 74103 | 5.02 | 4-7 | T | Facilitates the assembly of multimeric protein complexes inside the ER. |
| 73240 | 5.00 | 4-7 | T | |||
| Destrin | P18282 | 15816 | 6.28 | 4-7 | B | Actin-depolymerizing protein. |
| Myosin regulatory light chain 2 | P24844 | 19452 | 4.68 | 4-7 | -2.29 | Plays an important role in cytoskeletal regulation. |
| ERp29 (precursor) | P30040 | 27238 | 5.82 | 6-11 | 2.08 | Plays an important role in the processing of secretory proteins within the ER. |
| RKIP | P30086 | 20527 | 7.35 | 6-11 | B | Regulates Raf-1. |
| Mannose-6-phosphate isomerase | P34949 | 42747 | 5.46 | 4-7 | T | Glycosylation; early steps of mannosylation. |
| 14-3-3-γ | P35214 | 27834 | 4.74 | 4-7 | T | Inhibits PKC in platelets. |
| Transgelin 2 | P37802 | 20527 | 7.35 | 6-11 | B | Participates in cytoskeletal regulation pathways. |
| 21313 | 6.75 | 4-7 | 2.02 | |||
| α-Synuclein | P37840 | 18348 | 4.69 | 4-7 | -2.4 | Molecular chaperone. |
| Proteasome subunit β type 3 | P49720 | 21807 | 5.91 | 4-7 | -2.9 | Proteasome pathway. |
| Phenol-sulfating phenol sulfotransferase 1 | P50225 | 29941 | 5.93 | 4-7 | B | Catalyses the sulfate conjugation of catecholamines. |
| ILK-2 | P57043 | 50288 | 7.67 | 6-11 | 2.44 | Receptor-proximal protein kinase regulating integrin-mediated signal transduction |
| Autoantigen protein (fragment) | Q16081 | 91999 | 5.03 | 4-7 | T | Unknown |
| Ubiquitin-conjugating enzyme E2 N | Q16781 | 14522 | 5.72 | 4-7 | B | Catalyzes the covalent attachment of ubiquitin to other proteins. |
| 5′ Nucleotidase, deoxy (pyrimidine), cytosolic type C | Q8TCD5 | 24602 | 5.79 | 6-11 | 2 | Catalyzes the dephosphorylation of pyrimidine 5-prime monophosphates. |
| Hypothetical protein | Q96GF6 | 18726 | 5.79 | 4-7 | B | Possible phosphatase. Function unknown. |
| D-prohibitin | Q99623 | 31011 | 9.52 | 6-11 | B | Integral membrane protein, thought to regulate cation conductance. |
| RGS18 | Q9NS28 | 27711 | 6.94 | 6-11 | B | Inhibits signal transduction by increasing the GTPase activity of G protein α-subunits. |
| 27397 | 6.34 | 6-11 | 2.34 |
AN indicates SWISS-PROT accession number; pI, isoelectric point; B, protein feature only present in basal gels; RGS10, regulator of G-protein signaling 10; GTPase, guanosine 5′-triphosphatase; Dok-2, docking protein 2; HSP27, heat shock 27-kDa protein; T, feature only present in TRAP gels; ER, endoplasmic reticulum; ERp29, endoplasmic reticulum protein 29; RKIP, Raf kinase inhibitory protein; and ILK-2, integrin-linked protein kinase 2.
2-DE gel where the protein feature is present.
Fold change (TRAP versus basal), a negative value indicates that the feature is expressed to a higher extent in basal samples.
Function either known or predicted by homology via SWISS-PROT and NCBI.