Forced expression of C/EBPα induces differentiation of 32D cells expressing FLT3/ITD
Cell lines and treatment . | Myeloblasts, % . | Intermediates, % . | Granulocytes, % . |
|---|---|---|---|
| 32D/a-ER/FLT3/ITD | |||
| Before estradiol treatment | 88.7 ± 1.5 | 11.3 ± 1.5 | 0 |
| 2 d estradiol treatment | 0 | 47.7 ± 3.5 | 52.3 ± 3.5 |
| 32D/FLT3/ITD | |||
| Before estradiol treatment | 89 ± 3.6 | 11 ± 3.6 | 0 |
| 2 d estradiol treatment | 89.7 ± 3.7 | 10.3 ± 3.7 | 0 |
Cell lines and treatment . | Myeloblasts, % . | Intermediates, % . | Granulocytes, % . |
|---|---|---|---|
| 32D/a-ER/FLT3/ITD | |||
| Before estradiol treatment | 88.7 ± 1.5 | 11.3 ± 1.5 | 0 |
| 2 d estradiol treatment | 0 | 47.7 ± 3.5 | 52.3 ± 3.5 |
| 32D/FLT3/ITD | |||
| Before estradiol treatment | 89 ± 3.6 | 11 ± 3.6 | 0 |
| 2 d estradiol treatment | 89.7 ± 3.7 | 10.3 ± 3.7 | 0 |
Differential counts of 32D/α-ER/FLT3/ITD and 32D/FLT3/ITD cell cultures induced by estradiol were performed on day 0 and 2. Values are the mean percentages ± SD of cells from 3 independent counts examined by light microscopy after Wright-Giesma staining of the cytospins. “Intermediates” include promyelocytes, myelocytes, and metamyelocytes.